In vitro enrichment of ovarian cancer tumor-initiating cells

Journal of Visualized Experiments : JoVE
Carrie D HouseChristina M Annunziata

Abstract

Evidence suggests that small subpopulations of tumor cells maintain a unique self-renewing and differentiation capacity and may be responsible for tumor initiation and/or relapse. Clarifying the mechanisms by which these tumor-initiating cells (TICs) support tumor formation and progression could lead to the development of clinically favorable therapies. Ovarian cancer is a heterogeneous and highly recurrent disease. Recent studies suggest TICs may play an important role in disease biology. We have identified culture conditions that enrich for TICs from ovarian cancer cell lines. Growing either adherent cells or non-adherent 'floater' cells in a low attachment plate with serum free media in the presence of growth factors supports the propagation of ovarian cancer TICs with stem cell markers (CD133 and ALDH activity) and increased tumorigenicity without the need to physically separate the TICs from other cell types within the culture. Although the presence of floater cells is not common for all cell lines, this population of cells with innate low adherence may have high tumorigenic potential.Compared to adherent cells grown in the presence of serum, TICs readily form spheres, are significantly more tumorigenic in mice, and expres...Continue Reading

Citations

Aug 26, 2016·BMC Cancer·Marianne K KimChristina M Annunziata
Jan 8, 2018·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Aline Brito de LimaLetícia da Conceição Braga
Nov 28, 2019·Genome Biology·Jason K SaJeong-Won Lee
Aug 25, 2019·Cancers·Brittney S Harrington, Christina M Annunziata

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BETA
flow cytometry
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