In vitro evaluation of a novel bacteriophage cocktail as a preventative for bovine coliform mastitis

Journal of Dairy Science
J PorterM Paros

Abstract

The objective of this study was to investigate the potential use of bacteriophage in preventing Escherichia coli mastitis on dairies. A cocktail consisting of 4 distinct bacteriophages was generated by screening against 36 E. coli isolates from dairy cows in Washington State with clinical mastitis. The bacteriophage significantly inhibited growth of 58% of the Washington State isolates and 54% of E. coli mastitis isolates from New York State, suggesting that the cocktail of phages had a relatively broad spectrum of action against relevant strains from 2 distinct geographies. The ability to suppress bacterial growth of these isolates in a liquid growth medium was not affected by the ratio of bacteriophage particles to bacterial cells (multiplicity of infection, MOI). For those E. coli that were completely inhibited by the phage cocktail, an MOI as low as 10 had the same effect as 10 µg/mL of ceftiofur on the growth rate of E. coli over a 12-h period using optical density measurements. A 3.3- to 5.6-log reduction of growth was achieved when E. coli was co-incubated with our phage cocktail in raw milk over a 12-h period at physiologic temperature. A modified gentamicin protection assay using bovine mammary epithelial cells provide...Continue Reading

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Citations

Oct 11, 2016·Applied Biochemistry and Biotechnology·Krupa M ParmarNishant A Dafale
Apr 8, 2019·Journal of Dairy Science·Gunnar DalenOlav Reksen
May 23, 2020·Nutrition Reviews·Lisa F StinsonDonna T Geddes
May 3, 2019·Reviews in Medical Virology·Sana RehmanSaadia Naseem
Aug 14, 2019·Critical Reviews in Microbiology·Angeliki AngelopoulouR Paul Ross
Aug 12, 2020·Asian-Australasian Journal of Animal Sciences·Wei Nee Cheng, Sung Gu Han
Jan 30, 2021·The Veterinary Quarterly·Khan SharunWanpen Chaicumpa
Dec 29, 2020·Journal of Dairy Science·Wenpeng ZhaoJian Gao
Jun 20, 2018·Microbiology Spectrum·David H Lloyd, Stephen W Page
Apr 6, 2021·Critical Reviews in Microbiology·Avijit GoswamiRachit Agarwal

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