Abstract
Bone morphogenetic protein 9 (BMP9) has previously been characterized as the strongest osteoinductive growth factor among the BMP family. The aim of the present study was to evaluate the possibility of combining rhBMP9 with an injectable biphasic calcium phosphate (I-BCP, maxresorb inject®), since I-BCP is an easy to handle biomaterial with ideal properties for bone augmentation procedures. The adsorption potential of rhBMP9 as well as the cell behavior of bone stromal ST2 cells were investigated on cell viability, adhesion, proliferation and osteogenic differentiation for I-BCP combined with/without rhBMP9 in vitro. I-BCP demonstrated excellent adsorption/retention potential of rhBMP9 with a slow and steady release over a 10 day period by ELISA. Cell attachment at 8 hours and cell proliferation at 1, 3 and 5 days was decreased on I-BCP with/without rhBMP9 when compared to control tissue-culture plastic. While I-BCP had little influence on osteoblast differentiation, its combination with rhBMP9 significantly increased ALP activity at 7 days and mRNA levels of osteoblast differentiation markers including ALP and osteocalcin at 14 days. I-BCP served as an excellent carrier for rhBMP9 clearly demonstrating its osteoinductive poten...Continue Reading
References
Oct 1, 1995·Endocrinology·J J SongR S Thies
Oct 30, 1998·Biomaterials·G Daculsi
Jun 13, 2000·The Journal of Biological Chemistry·A F MillerM S Olson
Aug 20, 2003·The Journal of Bone and Joint Surgery. American Volume·Hongwei ChengTong-Chuan He
Jul 23, 2004·Gene Therapy·Q KangT-C He
Dec 29, 2004·Biomaterials·T Livingston ArinzehG Daculsi
Oct 19, 2006·Spine·John R DimarLeah Y Carreon
Feb 10, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hue H LuuTong-Chuan He
Feb 27, 2007·The Spine Journal : Official Journal of the North American Spine Society·Brian PerriNeel Anand
Sep 28, 2007·Journal of Biomedical Materials Research. Part a·C SchopperH Redl
Aug 20, 2009·Biotechnology Letters·Ziyad S HaidarMaryam Tabrizian
Mar 2, 2010·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·J C RoldánU Deisinger
May 25, 2010·Neurosurgery·Mark P GarrettVolker K H Sonntag
Jul 16, 2010·The Journal of Biological Chemistry·Jinyong LuoTong-Chuan He
Sep 24, 2011·Journal of Dental Research·R J MironD Buser
Feb 10, 2012·Journal of Dental Research·R J Miron, Y F Zhang
Apr 27, 2013·Clinical Oral Investigations·Richard J MironAnton Sculean
Jan 7, 2014·Journal of Dental Research·A C CarreiraJ M Granjeiro
Oct 11, 2014·Journal of Neurosurgery. Pediatrics·Brandon G RocquePaul A Anderson
Jan 17, 2015·Journal of Periodontology·Richard J MironAnton Sculean
Aug 19, 2015·Journal of Clinical Periodontology·Kosaku SawadaReinhard Gruber
Jan 12, 2016·Journal of Periodontology·Masako Fujioka-KobayashiRichard J Miron
Mar 19, 2016·Clinical Oral Implants Research·Masako Fujioka-KobayashiRichard J Miron
Oct 25, 2016·Clinical Oral Investigations·Masako Fujioka-KobayashiRichard J Miron
Citations
Apr 11, 2019·Cells·Ewa AmbrożewiczElżbieta Skrzydlewska
Jan 2, 2021·International Journal of Environmental Research and Public Health·Marija ČandrlićMarko Matijević
Jun 3, 2021·Materials·Matej TomasŽeljka Perić Kačarević