In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation

Biomedical Materials
Danielle Las AmaralMichele Munk

Abstract

Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering. In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation. In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC). Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays. Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity. The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation. Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold. Thus, the BT NP/algin...Continue Reading

References

Apr 15, 2003·Journal of Cell Science·Ana I TeixeiraPaul F Nealey
Nov 27, 2009·Nature·Matthias P LutolfHelen M Blau
Feb 8, 2011·PloS One·Cristina GamellFrancesc Ventura
Sep 26, 2012·Colloids and Surfaces. B, Biointerfaces·Gianni CiofaniVirgilio Mattoli
Feb 28, 2013·ACS Nano·Habib NikukarMatthew J Dalby
Dec 18, 2013·Acta Biomaterialia·Sílvia J BidarraPedro L Granja
Jan 23, 2014·Tissue Engineering. Part a·Laurent-Emmanuel MonfouletDelphine Logeart-Avramoglou
May 23, 2014·Nature Materials·Matthew J DalbyRichard O C Oreffo
May 28, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Yan ZhangDou Zhang
Jul 16, 2014·International Journal of Biological Macromolecules·Jayachandran VenkatesanSe-Kwon Kim
Apr 25, 2015·Journal of Cell Science·Werner E G MüllerXiaohong Wang
Feb 4, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Beilei LiuDou Zhang
Feb 10, 2016·Tissue Engineering. Part B, Reviews·Aaron W JamesChia Soo
Feb 19, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wenfeng XuXiaoling Liao
Apr 27, 2016·World Journal of Stem Cells·Susumu KohnoChiaki Takahashi
Apr 29, 2016·Nature Methods·Steven R Caliari, Jason A Burdick
Jun 2, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Rafael Serra-GómezGustavo González-Gaitano
Jul 9, 2016·ACS Nano·Elie Ngandu MpoyiManuel Salmerón-Sánchez
Feb 14, 2017·Nano Convergence·Sy-Tsong Dean ChuengKi-Bum Lee
Jun 24, 2017·Biofabrication·Tuğrul Tolga DemirtaşMenemşe Gümüşderelioğlu
Oct 6, 2017·Scientific Reports·Ilaria E PalamàBarbara Cortese
Nov 25, 2017·Nanomedicine : Nanotechnology, Biology, and Medicine·Diana R PereiraManus J Biggs
Dec 5, 2017·Materials Science & Engineering. R, Reports : a Review Journal·Jeroen LeijtenAli Khademhosseini
Dec 7, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Xingchen YangJinmin Zhao
Mar 7, 2018·Biomedical Materials·Catherine R LambertRyan G Wylie
Oct 3, 2018·ACS Applied Materials & Interfaces·Yang ZhangJeroen J J P van den Beucken
Feb 2, 2019·The Journal of Hand Surgery·David C LobbA Bobby Chhabra
Aug 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Min BaoYanzhong Zhang

❮ Previous
Next ❯

Citations

Jul 20, 2019·Journal of Biomaterials Science. Polymer Edition·Rafaella S S ZanetteMichele Munk

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.