In vitro evaluation of combination antifungal activity against Fusarium species isolated from ocular tissues of keratomycosis patients

American Journal of Ophthalmology
Li LiXuguang Sun

Abstract

To determine the minimum inhibitory concentrations (MICs) of five antifungal agents against Fusarium species isolated from ocular tissues and to evaluate anti-Fusarium species activities of eight combination treatments in vitro. Experimental research. Thirty-eight isolates of Fusarium species were collected from patients' ocular tissues and were cultured in vitro. The MICs of natamycin, terbinafine, itraconazole fluconazole, and amphotericin B, either used alone or combined with other compounds, were evaluated by checkerboard microdilution technique based on the Clinical Laboratory Standards Institute proposed standard. The interactions were assessed using the Fractional Inhibitory Concentration Index model. In the MIC study, the MIC(90) of each drug used alone were: natamycin, 16 microg/ml; terbinafine, 8 microg/ml; itraconazole, >16 microg/ml; fluconazole, >64 microg/ml; and amphotericin B, 4 microg/ml. Synergism was obtained in the amphotericin B plus terbinafine (81.6%) group and in the amphotericin B plus itraconazole (84.2%) group, with an obviously decreased MIC value of amphotericin B. Antagonism was shown in the natamycin plus azoles and in the natamycin plus terbinafine groups in 52.6% to 60.5% of Fusarium species str...Continue Reading

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Citations

Jan 25, 2013·Cornea·Rafael A OechslerEduardo C Alfonso
Oct 21, 2009·Antimicrobial Agents and Chemotherapy·Yan XuBingliang Wang
Jan 1, 2009·Clinical, Cosmetic and Investigational Dermatology·Jason G Newland, Susan M Abdel-Rahman
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Oct 31, 2009·Expert Opinion on Pharmacotherapy·S Krishnan-Natesan

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