In vitro evaluation of different methods of handling human liposuction aspirate and their effect on adipocytes and adipose derived stem cells

Journal of Cellular Physiology
Paola PalumboMaria Grazia Cifone

Abstract

Nowadays, fat tissue transplantation is widely used in regenerative and reconstructive surgery. However, a shared method of lipoaspirate handling for ensuring a good quality fat transplant has not yet been established. The study was to identify a method to recover from the lipoaspirate samples the highest number of human viable adipose tissue-derived stem cells (hADSCs) included in stromal vascular fraction (SVF) cells and of adipocytes suitable for transplantation, avoiding an extreme handling. We compared the lipoaspirate spontaneous stratification (10-20-30 min) with the centrifugation technique at different speeds (90-400-1500 × g). After each procedure, lipoaspirate was separated into top oily lipid layer, liquid fraction, "middle layer", and bottom layer. We assessed the number of both adipocytes in the middle layer and SVF cells in all layers. The histology of middle layer and the surface phenotype of SVF cells by stemness markers (CD105+, CD90+, CD45-) was analyzed as well. The results showed a normal architecture in all conditions except for samples centrifuged at 1500 × g. In both methods, the flow cytometry analysis showed that greater number of ADSCs was in middle layer; in the fluid portion and in bottom layer was ...Continue Reading

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Citations

Dec 10, 2015·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·A Jorien TuinJohan Jansma
Mar 11, 2016·Aesthetic Surgery Journal·Elizabeth R ZielinsDerrick C Wan
May 28, 2019·International Journal of Molecular Sciences·Loubna MaziniGabriel Malka
Feb 25, 2017·Plastic and Reconstructive Surgery·Libor StreitAles Hampl
May 13, 2017·International Journal of Molecular Sciences·Francesco De FrancescoGiuseppe A Ferraro
Jul 1, 2018·International Journal of Molecular Sciences·Paola PalumboMaurizio Giuliani
May 16, 2018·Clinical and Experimental Otorhinolaryngology·Do-Youn KimYong Ju Jang
Aug 8, 2019·International Journal of Molecular Sciences·Yoojin SeoHyung-Sik Kim
Jul 1, 2020·Journal of Tissue Engineering and Regenerative Medicine·Sheila VeroneseAndrea Sbarbati
Jan 6, 2021·Aesthetic Plastic Surgery·Yan LinDali Mu
Dec 19, 2020·Aesthetic Surgery Journal·Xiaonan YangJeffrey A Gusenoff
Mar 29, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zizhen SiYang Hui

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