PMID: 9546237Apr 18, 1998Paper

In vitro evaluation of electrostatic endothelial cell transplantation onto 4 mm interior diameter expanded polytetrafluoroethylene grafts

Journal of Vascular Surgery
G L BowlinS P Schmidt

Abstract

To perform an in vitro evaluation of electrostatic endothelial cell transplantation of human umbilical vein endothelial cells (HUVEC) onto segments of 4 mm internal diameter expanded polytetrafluoroethylene (ePTFE) vascular prostheses. This evaluation consisted of exposing vascular graft segments that had been subjected to either electrostatic or gravitation transplantation with HUVEC to a physiologic shear stress (15 dynes/cm2) under steady flow conditions within a flow loop system. Biochemical assays were performed on freshly transplanted grafts by means of radioimmunoassay for prostacyclin and thromboxane A2. There was a 30% loss of HUVEC after 30 minutes of shear stress exposure from the grafts subjected to gravitational transplantation with no additional significant (alpha = 0.05) loss after 120 minutes. Grafts subjected to electrostatic transplantation had no significant (alpha = 0.05) loss of HUVEC during exposure to physiologic shear stress. Furthermore, after 120 minutes of shear-stress exposure, the grafts subjected to electrostatic transplantation (78,420 +/- 6274 HUVEC/cm2) retained 2.3 times more HUVEC than the counterparts subjected to gravitational transplantation (34,427 +/- 4637 HUVEC/cm2). The biochemical assa...Continue Reading

References

Jun 1, 1987·Journal of Biomedical Materials Research·P B van WachemW G van Aken
Nov 1, 1985·Journal of Vascular Surgery·J E RosenmanE B Silberstein
May 1, 1981·The Journal of Clinical Investigation·C Ingerman-WojenskiE Macarak

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Citations

Dec 16, 2006·Endothelium : Journal of Endothelial Cell Research·Michel R HoenigJulie H Campbell
Aug 9, 2005·Cardiology Clinics·Prabir Roy-ChaudhurySue C Heffelfinger
May 22, 2007·Advanced Drug Delivery Reviews·Ge Zhang, Laura J Suggs
Sep 12, 2002·Journal of Biomaterials Applications·Charles FieldsMichael Szycher
Jan 4, 2005·Blood Purification·Prabir Roy-ChaudhurySue C Heffelfinger

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