In vitro evaluation of experimental agents for anti-HIV activity

Current Protocols in Immunology
D D RichmanH Mitsuya

Abstract

This unit presents an assay that has proven useful as an initial screening test is an HIV cytopathic effect (CPE) inhibition assay in which immortalized T cell lines (e.g., ATH8 or MT2) that are profoundly sensitive to the cytopathic effect of certain strains of HIV are utilized as target cells. Additional protocols assess the anti-HIV activity of certain candidate agents by measuring inhibition of syncytium formation or p24 gag protein production by ELISA. Calculation of the 50% inhibitory concentration (IC(50)) is also presented.

References

Feb 1, 1992·AIDS Research and Human Retroviruses·E De Clercq
Jan 1, 1992·International Journal of Clinical & Laboratory Research·J P Johnson
Sep 28, 1990·Science·H MitsuyaS Broder
Jul 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H MitsuyaS Broder
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D RichmanJ Griffin
Jan 1, 1990·Annals of the New York Academy of Sciences·V A Johnson, M S Hirsch
Jul 1, 1990·Reviews of Infectious Diseases·D D Richman
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T ShirasakaH Mitsuya
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E S DaarD D Ho
Nov 14, 1985·The New England Journal of Medicine·P S SarinA S Lippa
Aug 1, 1988·Journal of Virological Methods·R PauwelsE De Clercq
Feb 9, 1989·Nature·D J CaponJ E Groopman
Jul 11, 1986·Science·S GartnerM Popovic
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J E DahlbergS A Aaronson
May 28, 1993·Science·M I Johnston, D F Hoth
Jun 1, 1993·Antimicrobial Agents and Chemotherapy·D D Richman
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T ShirasakaH Mitsuya

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