In vitro evaluation of Ficoll-enriched and genipin-stabilised collagen scaffolds

Journal of Tissue Engineering and Regenerative Medicine
A SatyamD I Zeugolis

Abstract

Polysaccharides are frequently incorporated into scaffolds for tissue engineering applications to improve mechanical and biological properties. We evaluated the influence of a Ficoll® scaffold on collagen films, a scaffold that is extensively used for soft and hard tissue repair. To avoid cytotoxicity issues associated with chemical reagents, the influence of genipin, a naturally occurring crosslinking agent, was assessed. Ultra-structural level collagen films formed with and without Ficoll showed a fine fibrillar structure whereas genipin crosslinked films showed a coarse fibrillar and partially nodular structure. In contrast, glutaraldehyde crosslinked films lost their fibrillar pattern. Crosslinking significantly increased denaturation temperature (p < 0.001), stress (p < 0.0001) and force (p < 0.0001) at break. Collagen/Ficoll and collagen/Ficoll/genipin films showed the highest WI38 fibroblast attachment than any other scaffold (p < 0.003) and significantly greater WI38 fibroblast metabolic activity than other scaffolds (p < 0.001). By day 6. collagen/Ficoll/genipin films also induced higher and more aligned fibronectin matrix deposition than other scaffolds. Overall, this study indicates the suitability of collagen/Ficoll...Continue Reading

References

Jan 1, 1992·Biomaterials·M F Côté, C J Doillon
Sep 20, 1985·Journal of Molecular Biology·K M Meek, J A Chapman
Oct 21, 1968·Biochimica Et Biophysica Acta·J H Bowes, C W Cater
May 1, 1994·Journal of Biomedical Materials Research·M A MooreB K McIlroy
Feb 1, 1994·Journal of Biomedical Materials Research·H PetiteD Herbage
Apr 1, 1996·Biomaterials·L H Olde DaminkJ Feijen
Oct 1, 1996·Journal of Biomedical Materials Research·M A MooreB K McIlroy
Jan 1, 1996·Biotechnology & Genetic Engineering Reviews·J A RamshawV Glattauer
Jun 25, 1998·Journal of Biomedical Materials Research·D P KnightM W Ho
Aug 15, 1998·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·W Friess
Sep 2, 1998·Journal of Structural Biology·P FratzlS Bernstorff
Nov 25, 1998·Journal of Biomedical Materials Research·H W SungC T Chiu
Nov 25, 1998·Journal of Biomedical Materials Research·L L HuangD M Huang
Aug 17, 1999·Journal of Biomedical Materials Research·P B van WachemM J van Luyn
Nov 15, 2000·Journal of Biomedical Materials Research·V Charulatha, A Rajaram
Sep 13, 2001·Journal of Biomedical Materials Research·A K AdamsM A Moore
Feb 7, 2002·Biomaterials·Cécile F Rousseau, Christian H Gagnieu
Jun 14, 2003·TheScientificWorldJournal·R Gordon Paul, Allen J Bailey
Jun 5, 2004·International Journal of Biological Macromolecules·Alina Sionkowska, Tim Wess
Sep 16, 2004·Journal of Biomedical Materials Research. Part a·Kevin G CornwellGeorge D Pins
Sep 24, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·A K LynnW Bonfield
Oct 12, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Tero A H JärvinenMarkku Järvinen
Jul 12, 2005·Biomacromolecules·Gianluca CiardelliPaolo Giusti

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Citations

Jan 11, 2017·Tissue Engineering. Part a·Luis M DelgadoDimitrios I Zeugolis
Aug 21, 2018·Advanced Materials·Anna SorushanovaDimitrios I Zeugolis
Feb 16, 2018·Nature Protocols·Héctor Capella-MonsonísDimitrios I Zeugolis
Mar 25, 2014·Expert Review of Medical Devices·Luis M DelgadoDimitrios I Zeugolis
Sep 24, 2014·Biofabrication·Eric S HaldPatrick W Alford
Feb 2, 2021·Frontiers in Medicine·Nataly Puerta CavanzoRuud A Bank
May 15, 2015·Bioconjugate Chemistry·Christina N M RyanDimitrios I Zeugolis

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