PMID: 9189822Jun 15, 1997Paper

In vitro evaluation of heparinized Cuprophan hemodialysis membranes

Journal of Biomedical Materials Research
W L HinrichsJ Feijen

Abstract

Cuprophan hemodialysis membranes can be heparinized using N,N'-carbonyldiimidazole (CDI) as a coupling agent. In this study, the characteristics of heparinized Cuprophan membranes have been evaluated. After immobilization, heparin partially retained its biologic activity. An anticoagulant activity of 12.4 +/- 4.2 mU/cm2 was measured using a thrombin inactivation assay. Immobilized heparin also displayed an anti-complement activity. After contact with human serum; heparinized Cuprophan induced no generation of significant amounts of fluid phase terminal complement complex (TCC), whereas untreated Cuprophan induced the generation of substantial amounts of TCC. Heparinization did not affect the permeability of Cuprophan for model solutes with molecular weights up to 12,000 g/mol except for sulfobromophthalein sodium salt. The permeability of Cuprophan for sulfobromophthalein sodium salt was slightly decreased after heparinization. The ultrafiltration rate of Cuprophan increased by about 30% after heparinization, probably owing to an increased swelling of the membrane in water. Heparinized Cuprophan incubated in phosphate-buffered saline at 37 degrees C showed some release of heparin. These amounts of released heparin, however, wer...Continue Reading

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Citations

Mar 13, 2012·Cell Transplantation·Michael R DoranLars K Nielsen
Dec 31, 2011·Biotechnology Letters·Roger A NassarAlexander M Klibanov
Apr 10, 2019·Polymers·Marina VoinovaLeonid Gorelik
Jul 23, 2009·Tissue Engineering. Part C, Methods·Michael R DoranJustin J Cooper-White
Sep 8, 2001·Biomaterials·T Groth, W Wagenknecht
Jan 15, 2021·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Himanshu Patel

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