Abstract
Cuprophan hemodialysis membranes can be heparinized using N,N'-carbonyldiimidazole (CDI) as a coupling agent. In this study, the characteristics of heparinized Cuprophan membranes have been evaluated. After immobilization, heparin partially retained its biologic activity. An anticoagulant activity of 12.4 +/- 4.2 mU/cm2 was measured using a thrombin inactivation assay. Immobilized heparin also displayed an anti-complement activity. After contact with human serum; heparinized Cuprophan induced no generation of significant amounts of fluid phase terminal complement complex (TCC), whereas untreated Cuprophan induced the generation of substantial amounts of TCC. Heparinization did not affect the permeability of Cuprophan for model solutes with molecular weights up to 12,000 g/mol except for sulfobromophthalein sodium salt. The permeability of Cuprophan for sulfobromophthalein sodium salt was slightly decreased after heparinization. The ultrafiltration rate of Cuprophan increased by about 30% after heparinization, probably owing to an increased swelling of the membrane in water. Heparinized Cuprophan incubated in phosphate-buffered saline at 37 degrees C showed some release of heparin. These amounts of released heparin, however, wer...Continue Reading
References
Nov 1, 1979·The Journal of Experimental Medicine·M D KazatchkineK F Austen
Jan 1, 1992·Biomaterials·B MontdargentM Jozefowicz
Feb 1, 1992·Journal of the American Society of Nephrology : JASN·A K CheungE Brynda
Feb 1, 1990·Kidney International·R DeppischE W Rauterberg
Apr 1, 1987·Artificial Organs·A MahioutU Baurmeister
Jan 1, 1988·Advances in Experimental Medicine and Biology·A van Berlo, D J Ellens
Jun 1, 1988·Journal of Biomedical Materials Research·W L ChandlerG Schmer
Jul 1, 1986·Kidney International·A K CheungL W Henderson
Jan 1, 1986·Blood Purification·D L LevettD N Kerr
Sep 1, 1969·Journal of Biomedical Materials Research·J C BokrosM D Ramos
Oct 1, 1983·Biomaterials·E SchmittJ M Courtney
Dec 1, 1983·Kidney International·P IvanovichD E Hammerschmidt
Apr 1, 1982·Thrombosis Research·C D Ebert, S W Kim
Dec 1, 1980·Analytical Biochemistry·P K SmithG T Hermanson
Dec 1, 1980·Thrombosis Research·M F GoosenM W Hatton
Jan 1, 1993·Journal of Biomaterials Science. Polymer Edition·M Amiji, K Park
Feb 1, 1993·Biomaterials·M PeknaB Nilsson
Nov 1, 1995·Journal of Biomedical Materials Research·C J van DeldenJ Feijen
Jun 1, 1992·Artificial Organs·V V SinitsynV V Kukharchuk
Citations
Mar 13, 2012·Cell Transplantation·Michael R DoranLars K Nielsen
Dec 31, 2011·Biotechnology Letters·Roger A NassarAlexander M Klibanov
Apr 10, 2019·Polymers·Marina VoinovaLeonid Gorelik
Jul 23, 2009·Tissue Engineering. Part C, Methods·Michael R DoranJustin J Cooper-White
Sep 8, 2001·Biomaterials·T Groth, W Wagenknecht
Jan 15, 2021·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Himanshu Patel