In vitro evaluation of marrow clot enrichment on microstructure decoration, cell delivery and proliferation of porous titanium scaffolds by selective laser melting three-dimensional printing

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Qingqiang YaoLi-ming Wang

Abstract

Titanium alloy is a clinically approved material for bone substitution. Although three-dimensional printing (3DP) fabrication technique can build up porous Ti scaffolds with the designed shape and microstructure, the biomechanical performance of 3DP Ti scaffolds still need to be improved to increase the reliability of osseointegration capacity. To address this issue, rabbit bone marrow clot (MC) is used to modify 3DP Ti scaffolds by stem cell delivery and microenvironment decoration inside the pores of these scaffolds. Moreover, 3DP Ti scaffolds were built up using selective laser melting, and 3DP MC-Ti scaffolds were constructed through the enrichment of MC with Ti scaffolds in vitro. Results demonstrated that the obtained 3DP Ti scaffolds in current study has an average modulus of elasticity (ME) at 1294.48 MPa with average yield strength of 33.154 MPa. For MC-Ti scaffolds, MC enrichment obstructs the pores of 3DP scaffolds due to the large amount of fibrin and erythrocytes and leads to a decrease in ratio of live cells at 1-week culture. Cell proliferation and osteogenic differentiation performance of MC-Ti scaffolds were promoted with porous recanalization in the later 3 weeks. After 2 weeks in vitro culture, fivefold of ce...Continue Reading

References

Jun 13, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Siyu NiWanyin Zhai
Sep 21, 2010·Acta Biomaterialia·M Tarik ArafatIan Gibson
Jul 23, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Byoung-Hyun MinJeong Ho Yoon
May 9, 2014·Biomaterials·Saber Amin YavariAmir Abbas Zadpoor
Nov 11, 2014·Journal of Tissue Engineering·Waldemar HoffmannDavid Wendt
Dec 17, 2014·Acta Biomaterialia·Ruben WauthleJan Schrooten
Jan 19, 2015·Journal of Materials Science. Materials in Medicine·Qingqiang YaoLiming Wang
Apr 12, 2015·Advanced Drug Delivery Reviews·Rachit Agarwal, Andrés J García
Jul 27, 2015·Journal of the Mechanical Behavior of Biomedical Materials·Chunze YanPhilippe Young
Sep 1, 2015·Acta Biomaterialia·Adam B NoverClark T Hung
Apr 3, 2016·Colloids and Surfaces. B, Biointerfaces·Bindu P NairPrabha D Nair

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.