In vitro evaluation of migratory capacity of human liver stem cells influenced by soluble factors

Transplantation Proceedings
I K JangS-K Lee

Abstract

Although several studies have addressed the engraftment of stem cells into the liver, the exact mechanisms in vivo remain unclear. In this study, we investigated the effects of soluble factors on cell migration using purified, expanded human liver stem cells (HLSCs) obtained from a pediatric liver resection. Using a in vitro transwell migration assay, we evaluated the migratory capacity of HLSCs under the influence of the cytokines tumor necross factor- [TNF]-α, interleukin [IL]-6, and interferon (IFN)-γ or the growth factors vascular endothelial growth factor [VEGF], basic fibroblast growth factor [bFGF], and hepatocyte growth factor [HGF], which are known to be highly secreted during liver injury. We also evaluated the migratory capacity indirectly influenced by cryopreserved human hepatocytes. The migration across the transwell membrane was promoted by VEGF, bFGF, TNF-α, IFN-γ, or hepatocytes. The cryopreserved human hepatocytes especially induced significant migration. These results suggested the presence of unidentified soluble factors from hepatocytes. This experiment described a reliable system for quantitative migration studies to broaden our understanding of the directional nature of cell migration.

References

Sep 2, 2006·Stem Cells·Maria Beatriz HerreraGiovanni Camussi
Jun 15, 2007·Journal of Cellular Physiology·George K Michalopoulos
Jun 29, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Agnieszka BanasTakahiro Ochiya
Jan 15, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Yongmin YanFei Mao
Jan 28, 2010·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Claire TerryRobin D Hughes

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