In vitro evaluation of nanosized carbonate-substituted hydroxyapatite and its polyhydroxyethylmethacrylate nanocomposite

Journal of Biomedical Materials Research. Part a
J HuangW Bonfield

Abstract

Nanometer scale carbonate-substituted hydroxyapatite (nanoCHA) particles were prepared and examined using transmission electron microscopy, which revealed their polycrystalline nature with a rod-like morphology (20-30 nm in width and 50-80 nm in length). In vitro cytotoxicity study showed that there was some evidence of lactate dehydrogenase (LDH) release when macrophages were in contact with high concentrations of nanoCHA particles. The levels of LDH release decreased significantly with a reduction in nanoCHA concentration. A similar trend was observed for the inflammatory cytokine TNF-alpha. nanoCHA particles with high carbonate content induced a high level of TNF-alpha release. Biological testing using a human osteoblast (HOB) cell model found that HOB cells were able to grow and proliferate on a nanoCHA deposited surface. Well organized actin fibers were observed for HOB cells in contact with nanoCHA particles with low carbonate content and the cell proliferation rate was higher on these particles in comparison with those of high carbonate nanoCHA particles. Therefore, low carbonate nanoCHA particles were incorporated into poly-(2-hydroxyethylmethacrylate) matrix to make a nanocomposite. It was found that the nanoCHA compos...Continue Reading

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Sep 7, 2004·Journal of Materials Science. Materials in Medicine·J HuangW Bonfield
May 19, 2005·Biomaterials·Felicia SuskaPeter Thomsen

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Citations

Aug 26, 2010·Tissue Engineering. Part B, Reviews·Katerina GkioniJohn A Jansen
Jan 31, 2014·Journal of the Royal Society, Interface·Priya KaliaJie Huang
Jan 18, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Jian Ping FanJie Huang
Jan 25, 2019·Biomaterials Research·Jiwoon JeongChan Yeong Heo
Mar 13, 2021·Advanced Materials·Bernette Maria OosterlakenGijsbertus de With
Oct 1, 2012·Materials Science & Engineering. C, Materials for Biological Applications·Łukasz JohnPiotr Dzięgiel
Nov 20, 2021·ACS Biomaterials Science & Engineering·Marjan BahraminasabMohammad Sadegh Nourbakhsh

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