In vitro evaluation of orthopedic composite cytotoxicity: assessing the potential for postsurgical production of hydroxyl radicals

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Michael DiCiccoSusan A Jansen-Varnum

Abstract

Hydroxyl radical (*OH)-induced inflammation is a primary mode for in vivo cytotoxicity. A legitimate concern is whether particulate wear debris from orthopedic composites can stimulate inflammation via ferrous ion (Fe2+)-mediated production of *OH. The purpose of this research was to utilize electron paramagnetic resonance (EPR) spin trapping in investigating and comparing the potential for postsurgical cytotoxicity induced specifically by *OH in the presence of two composites: Simplex P and the novel, hybrid, CORTOSS. Cytotoxicity is evaluated based on the composites competitively chelating catalytic Fe2+ or readily reducing ferric ions (Fe3+), in facilitating the Fenton reaction (FR). *OH that are produced were then validated by a radical scavenger to confirm a genuine radical signal and mechanism. Spin adduct peak areas decreased in the presence of CORTOSS as opposed to increasing in the presence of Simplex P, evaluated against their respective controls. A plausible theory elucidating this finding is that CORTOSS may sequester the Fe form, by virtue of its monomers. Principally, direct comparison of composites indicated that Simplex P had greater tendency to produce *OH, yielding 25.6 and 48.7% greater spin adduct peak areas...Continue Reading

References

Jul 1, 1979·Physiological Reviews·B ChanceA Boveris
Jan 1, 1990·Free Radical Biology & Medicine·D M MillerS D Aust
Apr 1, 1990·Archives of Biochemistry and Biophysics·L G Lund, A E Aust
Jan 1, 1989·Chemico-biological Interactions·E G JanzenJ W Hilborn
May 1, 1986·Archives of Biochemistry and Biophysics·B Halliwell, J M Gutteridge
May 1, 1986·Journal of Biomedical Materials Research·M A Looney, J B Park
Jan 1, 1980·Biomaterials, Medical Devices, and Artificial Organs·J B ParkP E Atkins
Jan 1, 1982·Critical Reviews in Toxicology·R P StankusJ E Salvaggio
Jan 5, 1995·Biochemical and Biophysical Research Communications·D GelvanP Saltman
Jan 10, 1995·Archives of Biochemistry and Biophysics·S K Eborn, A E Aust
Apr 1, 1994·Free Radical Biology & Medicine·L A ReinkeP B McCay
Jan 1, 1997·Free Radical Biology & Medicine·R V LloydR P Mason
Jul 9, 1999·Journal of Biomedical Materials Research·F F Oldfield, H K Yasuda
Feb 24, 2001·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·H E Jasin

❮ Previous
Next ❯

Citations

Oct 8, 2013·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Yang YiminRajiv Jha
Jun 16, 2010·The Journal of Oral Implantology·Wael Aly Ghuneim
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ali KhademhosseiniJoseph P Vacanti
Aug 7, 2010·Acta Biomaterialia·Johan Van der StokPeter Patka
Apr 7, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Z Y LiK D K Luk
Jan 25, 2008·The Journal of Craniofacial Surgery·Galip Zihni SanusFatma Ozlen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
E M ErbeG Gualtieri
Journal of Biomedical Materials Research
L L Hench, H A Paschall
Injury
Peter V GiannoudisEleftherios Tsiridis
© 2021 Meta ULC. All rights reserved