In vitro evaluation of pyridine-2-azo-p-dimethylaniline cephalosporin, a new diagnostic chromogenic reagent, and comparison with nitrocefin, cephacetrile, and other beta-lactam compounds.

Journal of Clinical Microbiology
R N JonesW J Novick

Abstract

Pyridine-2-azo-p-dimethylanaline cephalosporin (PADAC), a chromogenic reagent which is purple and changes to yellow upon cleavage of its beta-lactam ring, was evaluated in comparison with other chromogenic cephalosporins. PADAC exhibited little antimicrobial activity against gram-negative bacteria, but did have good activity (minimum inhibitory concentration, 0.12 to 0.5 microgram/ml) against Staphylococcus aureus, a quality comparable to nitrocefin. Nitrocefin, however, demonstrated an unexpected and uniquely potent activity against Streptococcus faecalis (minimum inhibitory concentration, less than or equal to 0.06 to 0.12 microgram/ml) The relative hydrolysis rate of PADAC when subjected to six different beta-lactamases was substantially greater than that of cephacetrile, but less than that of nitrocefin. The relative hydrolysis rates of PADAC and nitrocefin were comparable with type IIIa beta lactamase and the derived from Bacillus cereus. The inhibition of beta-lactamase hydrolysis of the chromogenic cephalosporin substrates by six enzyme-stable inhibitors was generally greater with PADAC than with nitrocefin. Unlike nitrocefin, PADAC mixed with 50% human serum or various broth culture media showed no evidence of color cha...Continue Reading

References

Dec 25, 1976·Lancet·A PercivalE H Annels
Aug 1, 1979·Antimicrobial Agents and Chemotherapy·H C NeuK Kung
Nov 1, 1976·Journal of Clinical Microbiology·R N JonesJ Bigelow
Feb 23, 1974·Lancet·W J ThomasD W Bailey
Apr 1, 1972·Antimicrobial Agents and Chemotherapy·C H O'CallaghanA H Shingler
Dec 1, 1972·Antimicrobial Agents and Chemotherapy·C O'Callaghan, A Morris
Dec 1, 1981·Antimicrobial Agents and Chemotherapy·R N JonesH W Wilson
Nov 1, 1974·Antimicrobial Agents and Chemotherapy·C Thornsberry, L A Kirven

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Citations

Aug 7, 2007·Angewandte Chemie·Hequan YaoJianghong Rao
Aug 20, 2020·Chemical Communications : Chem Comm·Dahlia AlkekhiaAnita Shukla
Jan 27, 1999·Nature Biotechnology·D LegendreJ Fastrez
Oct 30, 2013·ChemMedChem·Anne MakenaChristopher J Schofield
Oct 30, 2020·ChemistryOpen·Srikrishna TummalaYen-Peng Ho
Jan 1, 1991·Journal of General Microbiology·A Seoane, J M García Lobo
May 1, 1982·Journal of Clinical Microbiology·R N JonesC Thornsberry
Feb 1, 1983·Journal of Clinical Microbiology·A L BarryC Thornsberry
Jan 10, 2012·Journal of the American Chemical Society·Shin MizukamiKazuya Kikuchi
Aug 1, 2013·Journal of Medicinal Chemistry·Sander S van BerkelChristopher J Schofield

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