PMID: 7544687Aug 1, 1995Paper

In vitro evaluation of Pyrularia thionin-anti-CD5 immunotoxin

Cancer Immunology, Immunotherapy : CII
S E GasanovL P Vernon

Abstract

The membrane-active peptide, Pyrularia thionin, purified from Pyrularia pubera, was covalently conjugated to an anti-CD5 monoclonal antibody. The membrane-active properties of thionin were not affected by the conjugation. The immunotoxin killed CD5+ lymphocytes in vitro at a concentration of 0.1 nmol/10(7) cells after 2 h of incubation. The immunotoxin also inhibited the proliferation of T cells in vitro, stimulated either by mitogens or in the mixed lymphocyte reaction. It was shown by electron paramagnetic resonance of spin probes and differential scanning calorimetry that the ability of the immunotoxin to perturb the lipid phase of membranes is close to that of unconjugated thionin. The results obtained suggest that Pyrularia-thionin-anti-CD5 conjugate may be useful for graft-versus-host disease therapy and potentially in the treatment of CD5+ leukemia and lymphomas.

References

Jan 1, 1992·Pharmacology & Therapeutics·L P Vernon, J D Bell
Oct 1, 1986·Cellular Immunology·O W PressP J Martin
Jan 21, 1985·Journal of Immunological Methods·L H StankerH Juarez-Salinas
Apr 1, 1985·Archives of Biochemistry and Biophysics·L P VernonW R Gray
Sep 29, 1981·Biochemistry·Y AracavaS Schreier
Sep 1, 1993·American Journal of Surgery·C P Theuer, I Pastan
Mar 1, 1993·Archives of Biochemistry and Biophysics·S E GasanovT F Aripov

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Citations

Mar 10, 2009·Toxicon : Official Journal of the International Society on Toxinology·Mayra TejucaMauro Dalla Serra
May 20, 1999·Biopolymers·D Andreu, L Rivas
May 20, 2005·Gene Therapy·L Johannes, D Decaudin

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