In vitro evaluation of sun protection factors of sunscreen agents using a novel UV spectrophotometric technique

International Journal of Cosmetic Science
M D Bleasel, S Aldous

Abstract

A method for the in vitro determination of low- and high-value sun protection factors (SPF) of sunscreens using artificial substrates and a novel pseudo double beam (PDB) mode of operation of a standard double beam UV spectrophotometer is described. The method allows transmittance to be calculated from detector responses of reference and sample beams measured at different gain levels and facilitates the accurate quantification of low levels of electromagnetic radiation transmitted through highly absorbing samples. The spectrophotometer was modified to hold quartz diffusing plates on which a substrate [Transpore adhesive tape or human stratum corneum obtained from a skin surface biopsy (SSB)] and the sunscreens to be tested were applied. The PDB mode of operation increased the effective linear range of the detector response of the spectrophotometer by a factor of approximately 20000-fold, enabling the in vitro SPF determination technique to be applied to both high and low SPF value sunscreens. Eight commercial sunscreens with known SPF values ranging from 4 to 77, previously determined by in vivo methods, were tested in vitro using both test substrates and correlations between the in vivo and in vitro values were determined. SPF...Continue Reading

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Citations

Jul 15, 2015·Journal of Photochemistry and Photobiology. B, Biology·D BacquevilleH Duplan
Dec 12, 2012·ISRN Dermatology·Maria PelizzoMauro Alaibac
Apr 29, 2010·Current Oncology Reports·Pierre Giglio, Mark R Gilbert

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