In vitro evaluation of the quality and fertilizing capacity of boar semen frozen in 0.25 ml straws

Reproduction in Domestic Animals = Zuchthygiene
J PeláezJ C Domínguez

Abstract

Twenty-two boar ejaculates were frozen in 0.25 ml straws using a controlled cooling rate, then evaluated in vitro in order to assess: (i) the extent to which a range of semen evaluation parameters accurately characterize sperm quality, (ii) the value of quality assessment in the characterization of long-term sperm survival and fertility and (iii) the suitability of the cryopreservation protocol used for yielding semen with good quality and fertilizing capacity. Motility with or without caffeine, plasma membrane integrity (PMI) evaluated with both propidium iodide (PI) and Hoechst 33258, and acrosome morphology were studied, the ejaculates being then classified into five quality groups. A thermoresistance test and a homologous in vitro fertilization test were applied to selected ejaculates of these groups. Caffeine-stimulated motility and PMI evaluated with PI provided better estimations of semen quality than the other tests of motility, PMI, or acrosome morphology, but this quality assessment could not reveal differences in fertilizing capacity or thermoresistance among ejaculates. Over 43% spermatozoa survived cryopreservation in 19 of the 22 ejaculates, with inter-boar and inter-ejaculate variability in the freezing success b...Continue Reading

References

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Citations

May 20, 2008·Reproduction in Domestic Animals = Zuchthygiene·K BuranaamnuayM Techakumphu
Sep 21, 2006·Reproduction in Domestic Animals = Zuchthygiene·H Rodríguez-Martínez
Apr 14, 2015·Reproduction in Domestic Animals = Zuchthygiene·H H ShiomiJ D Guimarães
Jun 13, 2013·Theriogenology·Rocío Fernández-GagoFelipe Martínez-Pastor
Jun 10, 2016·Fertility and Sterility·Bjorn de WagenaarLoes I Segerink
Mar 15, 2008·Computers in Biology and Medicine·Enrique AlegreLidia Sánchez
Jul 4, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Tim Ireland, Simon Garnier
Jul 16, 2015·Reproduction in Domestic Animals = Zuchthygiene·R V Knox
Apr 24, 2007·Theriogenology·Hans EkwallHeriberto Rodríguez-Martínez

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