In vitro evaluation of the risk of inflammatory response after chitosan/HA and chitosan/β-1,3-glucan/HA bone scaffold implantation

Materials Science & Engineering. C, Materials for Biological Applications
A Przekora, G Ginalska

Abstract

The aim of the study was to evaluate in vitro the risk of inflammatory response induced by chitosan/hydroxyapatite (chit/HA) and novel chitosan/β-1,3-glucan/hydroxyapatite (chit/glu/HA) bone scaffolds. The inflammatory response was assessed via measurement of proinflammatory cytokine and ROI production by human monocytes, macrophages, and osteoblasts stimulated with investigated scaffolds. Moreover, adsorption of human serum/plasma proteins to the tested materials was determined. Both biomaterials did not induce intracellular ROI generation by monocytes, macrophages, and osteoblasts and did not stimulate proinflammatory cytokine (IL-6 and TNF-α) production by inflammatory cells. Moreover, the chit/glu/HA material induced increased TNF-α production by osteoblasts that is believed to enhance osteogenic differentiation. Thus, it was demonstrated that chit/HA and chit/glu/HA scaffolds carry a low risk of biomaterial-induced inflammatory response and are promising materials as bone scaffolds for bone tissue engineering and regenerative medicine applications.

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Oct 20, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Agata PrzekoraGrazyna Ginalska

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Citations

Jul 16, 2016·Journal of Biomaterials Science. Polymer Edition·Jeong Eun SongGilson Khang
Jul 25, 2019·Journal of Functional Biomaterials·Alistair LockDavid S Musson
May 12, 2018·Genes·Julia ScheinpflugFrank Schulze
Jan 28, 2021·International Journal of Molecular Sciences·Paulina KazimierczakAgata Przekora
Jan 30, 2021·International Journal of Biological Macromolecules·Ling DingJi Zhang
Feb 14, 2021·Polymers·Clément ToullecChristine Jérôme
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wataru AriyoshiRyota Yamasaki
May 11, 2021·Macromolecular Bioscience·Zhuqing Li, Kaitlin M Bratlie

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