In vitro evaluation of the role of platelet-activating factor and interleukin-8 in Mannheimia haemolytica-induced bovine pulmonary endothelial cell injury

American Journal of Veterinary Research
D J McClenahanDouglas J Weiss

Abstract

To develop an in vitro model of the bovine alveolar-capillary interface and to evaluate the roles of interleukin-8 (IL-8) and platelet-activating factor (PAF) in neutrophil-mediated endothelial injury induced by infection with Mannheimia haemolytica. Cultured bovine pulmonary microvascular endothelial cells, freshly isolated bovine neutrophils, and monocyte-derived bovine macrophages. A coculture system was developed in which endothelial cells were grown to confluence in tissue culture inserts, neutrophils were added to the inserts, and macrophages were added to tissue culture wells. Mannheimia haemolytica-derived lipopolysaccharide (LPS) or supernatant was added to activate macrophages, and inhibitors of PAF or IL-8 were added to the insert. Endothelial cell cytotoxicity and permeability (ie, albumin leakage) and neutrophil activation (ie, adhesion, degranulation [lactoferrin expression], and superoxide production) were assessed. The addition of M haemolytica-derived LPS to bovine macrophages in the coculture system resulted in significant increases in endothelial cell cytotoxicity and permeability and neutrophil degranulation and adhesion. Inhibition of IL-8 reduced endothelial cell permeability and neutrophil degranulation i...Continue Reading

References

May 1, 1987·Veterinary Microbiology·C J CzuprynskiE J Noel
Mar 1, 1973·The Journal of Clinical Investigation·B M BabiorJ T Curnutte
Apr 1, 1981·Veterinary Immunology and Immunopathology·J A Roth, M L Kaeberle
Jul 1, 1995·The American Journal of Physiology·D MacconiA Remuzzi
Aug 1, 1993·The Journal of Clinical Investigation·D E LorantG A Zimmerman
Jul 1, 1993·Veterinary Immunology and Immunopathology·K PerssonC Hallén Sandgren
May 1, 1993·Infection·Y LermanY L Danon
Feb 25, 1996·Cellular Immunology·J H WangD Bouchier-Hayes
Mar 1, 1996·The Journal of Surgical Research·R J FrancioseA Banerjee
Jun 1, 1996·The Journal of Surgical Research·P S DahlbergD L Dunn
Apr 1, 1996·Microbial Pathogenesis·M A MorseyL A Babiuk
Aug 1, 1996·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·E J MillerB T Peterson
Feb 15, 1997·Lancet·D H Adams, A R Lloyd
Mar 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·M Introna, A Mantovani
Nov 14, 1997·American Journal of Respiratory and Critical Care Medicine·L OchoaF L Minnear
Nov 22, 1997·The Veterinary Clinics of North America. Food Animal Practice·D Griffin
Feb 6, 1998·World Journal of Surgery·S N McGillN V Christou
Jun 10, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E A GillG A Zimmerman
Jun 10, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M K TophamG A Zimmerman
Jul 31, 1998·Clinical Science·A M CondliffeE R Chilvers
Feb 25, 1999·American Journal of Respiratory Cell and Molecular Biology·J PuginA F Suffredini
May 26, 1999·American Journal of Respiratory Cell and Molecular Biology·A J MackarelC M O'Connor
Aug 17, 1999·The Journal of Experimental Medicine·T VasselonP A Detmers
May 10, 2000·American Journal of Veterinary Research·D McClenahanD Weiss
Mar 14, 2001·American Journal of Physiology. Cell Physiology·H Lum, K A Roebuck

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Citations

Aug 6, 2010·Veterinary Pathology·K SinghA W Confer
Mar 26, 2009·Journal of the National Cancer Institute·Bonnie E Gould RothbergDavid L Rimm
Feb 16, 2018·Cell and Tissue Research·Laura L Bassel, Jeff L Caswell

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