In vitro evaluation on HeLa cells of protective mechanisms of probiotic lactobacilli against Candida clinical isolates

Journal of Applied Microbiology
Maria Magdalena ComanAlberto Cresci

Abstract

To characterize in vitro the ability of human Lactobacillus strains to inhibit the adhesion, to displace and to compete with clinically isolated Candida strains. Three types of assays were performed to determine the inhibitory effect of Lactobacillus plantarum 319, Lactobacillus rhamnosus IMC 501, Lactobacillus paracasei IMC 502 and a specific probiotic combination (SYNBIO) on adhesion of Candida pathogens to HeLa cells: blockage by exclusion (lactobacilli and HeLa followed by pathogens), competition (lactobacilli, HeLa and pathogens together) and displacement (pathogens and HeLa followed by the addition of lactobacilli). Bacterial adhesion to HeLa was quantified by microscopy after May-Grünwald/Giemsa stain. The inhibition results highlight a significant (P < 0·05) competition of the considered probiotics against all the Candida strains. The results suggest that the probiotic strains used in this study could prevent colonization of the urogenital tract by relevant pathogens such as Candida strains through barrier and interference mechanisms (mainly displacement and competition), but the degree of inhibition of adhesion was bacterial strain-dependent. The results support the potential of these Lactobacillus probiotic strains as...Continue Reading

References

Aug 7, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M M McNeilD W Warnock
May 17, 2002·The Journal of Hospital Infection·V Krcmery, A J Barnes
Aug 1, 2002·Journal of Clinical Microbiology·Alejandra VásquezGöran Molin
Sep 16, 2003·Journal of Medical Microbiology·Yuan-Kun LeeSeppo Salminen
Nov 1, 2003·The Lancet Infectious Diseases·Philippe EggimannDidier Pittet
Jan 30, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·T J WalshE Anaissie
Oct 19, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Scott K Fridkin
Sep 13, 2006·FEMS Immunology and Medical Microbiology·Jasadee KaewsrichanJaturavit Kongprasertkit
Jan 16, 2007·Clinical Microbiology Reviews·M A Pfaller, D J Diekema
Jan 10, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·B R Goldin, S L Gorbach
Mar 7, 2009·Food & Nutrition Research·Abiola C Senok
Jan 1, 2008·Interdisciplinary Perspectives on Infectious Diseases·Sarah CribbyGregor Reid
Apr 15, 2009·European Journal of Nutrition·Maria Cristina VerdenelliAlberto Cresci
Jun 16, 2011·Mycoses·Daniela Mailänder-SánchezMartin Schaller
Oct 31, 2013·Archives of Gynecology and Obstetrics·Sandra BorgesPaula Teixeira
Dec 5, 2013·Journal of Lower Genital Tract Disease·Aziz HomayouniElnaz Vaghef Mehrabany
Mar 27, 2015·Infectious Disorders Drug Targets·Umme HaniAtul Shrivastava

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Citations

Jun 18, 2017·Beneficial Microbes·N CalonghiB Vitali
Jan 29, 2019·FEMS Yeast Research·Priscilla Romina De GregorioMaría Elena Fátima Nader-Macías
Oct 2, 2019·Antimicrobial Agents and Chemotherapy·Marwa AlhashimiMohamed N Seleem
Jan 16, 2019·BMC Microbiology·Deirdre Elizabeth O'HanlonThomas R Moench
Jun 3, 2021·American Journal of Reproductive Immunology : AJRI·Janine JewanrajLenine J P Liebenberg
Jul 4, 2021·Journal of Applied Microbiology·Francesco M CarpiValerio Napolioni

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