In vitro evidence for chronic alcohol and high glucose mediated increased oxidative stress and hepatotoxicity

Alcoholism, Clinical and Experimental Research
Karthikeyan ChandrasekaranAparajita Dey

Abstract

Hyperglycemia or alcoholism can lead to impaired liver functions. Cytochrome P450 2E1 (CYP2E1) is elevated in hyperglycemia or alcoholism and plays a critical role in generating oxidative stress in the cell. In the present study, we have used VL-17A cells that overexpress the alcohol metabolizing enzymes [alcohol dehydrogenase (ADH) and CYP2E1] to investigate the toxicity due to ethanol (EtOH) plus high glucose. Toxicity was assessed through viability assay and amount of acetaldehyde adduct formation. Oxidative stress parameters included measuring reactive oxygen species (ROS) levels and malondialdehyde adduct formation. Apoptosis was determined through caspase-3 activity, Annexin V- Propidium iodide staining, and changes in mitochondrial membrane potential. The effects of antioxidants and specific inhibitors of ADH and CYP2E1 on cell viability and ROS levels were also studied. When present together, EtOH plus high glucose-treated VL-17A cells exhibited greater oxidative stress and toxicity than other groups. Apoptosis was observed in liver cells treated with the toxins, and the EtOH plus high glucose-treated VL-17A cells exhibited apoptosis to the largest extent. A distinct and graded increase in CYP2E1 level occurred in the d...Continue Reading

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Mar 26, 2016·Alcoholism, Clinical and Experimental Research·Srikar MunukutlaSuresh S Palaniyandi
Apr 23, 2015·Asian Pacific Journal of Tropical Medicine·Guang-Hao GeWei-Yi Fang
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Related Concepts

Acetaldehyde
Alcohol Dehydrogenase II
Ethanol
Antioxidant Effect
Cell Survival
Central Nervous System Depressants
Glucose, (beta-D)-Isomer
Sugar Substitutes
Apoptosis, Intrinsic Pathway
Reactive Oxygen Species

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis