In vitro evidence for T-B lymphocyte collaboration in the clawed toad, Xenopus

European Journal of Immunology
B BlombergLouis Du Pasquier

Abstract

Requirements for an in vitro secondary antibody response to the soluble antigens dinitrophenylated keyhole limpet hemocyanin and dinitrophenylated flow gamma-globulin are described for the clawed toad, Xenopus. Priming of both hapten and carrier-specific cells is required in order to obtain good responses to the hapten. The carrier-reactive ("helper") cells do not adhere to nylon wool, are X ray-resistant and surface Ig-negative. The cell adherence and X ray sensitivity characteristics of these Xenopus "T" cells and their ability to generate a mixed lymphocyte response, allogeneic cytotoxic and helper activities, are identical to those of thymus-derived (T) cells in higher vertebrates. The hapten-reactive, antibody-secreting cell precursors are nylon-adherent, X ray-sensitive and surface Ig-positive. In the absence of other distinguishing serological markers for T cells in frogs, and considering the thymus dependency of the helper activity described here, the evidence presented is interpreted as demonstrating the requirement for antigen-specific T-B cell cooperation in response to soluble protein antigens in vitro for Xenopus.

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Citations

Jan 1, 1985·Developmental and Comparative Immunology·L N RubenM Balls
Jan 1, 1987·Immunology Today·M F FlajnikL D Pasquier
Oct 8, 1999·Developmental and Comparative Immunology·C CareyL Rollins-Smith
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Jun 20, 2017·Virology·Robert JacquesDe Jesús Andino Francisco
Apr 27, 2019·Physics in Medicine and Biology·M ObeidatN Kirby
Feb 1, 1981·European Journal of Immunology·C C BernardL Du Pasquier
Nov 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shauna MarrJacques Robert
Mar 10, 2018·European Journal of Immunology·Jacques Robert

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