In vitro evidence that hyperglycemia stimulates tumor necrosis factor-alpha release in obese women with polycystic ovary syndrome

The Journal of Endocrinology
F GonzalezJohn P Kirwan

Abstract

Women with polycystic ovary syndrome (PCOS) are often insulin resistant and have chronic low-level inflammation. The purpose of this study was to determine the effects of hyperglycemia in vitro on tumor necrosis factor (TNF)-alpha release from mononuclear cells (MNC) in PCOS. Twelve reproductive-age women with PCOS (six lean, six obese) and 12 age-matched controls (six lean, six obese) were studied. Insulin sensitivity (IS(HOMA)) was estimated from fasting levels of glucose and insulin and percent truncal fat was determined by dual energy absorptiometry (DEXA). TNFalpha release was measured from MNC cultured under euglycemic and hyperglycemic conditions. IS(HOMA) was higher in obese women with PCOS than in lean women with PCOS (student's t-test; 73.7 +/- 14.8 vs 43.1 +/- 8.6, P < 0.05), but similar to that of obese controls. IS(HOMA) was positively correlated with percent truncal fat (r=0.57, P < 0.04). Obese women with PCOS exhibited an increase in the percent change in TNFalpha release from MNC in response to hyperglycemia compared with obese controls (10 mM, 649 +/- 208% vs 133 +/- 30%, P < 0.003; 15 mM, 799 +/- 347% vs 183 +/- 59%, P < 0.04). The TNFalpha response directly correlated with percent truncal fat (r=0.45, P < 0....Continue Reading

Citations

Jan 31, 2008·Expert Reviews in Molecular Medicine·Evanthia Diamanti-Kandarakis
Oct 8, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tomoya TerashimaLawrence Chan
Nov 3, 2011·American Journal of Physiology. Endocrinology and Metabolism·Frank GonzálezJill M Schimke
Dec 20, 2012·American Journal of Physiology. Endocrinology and Metabolism·Frank GonzálezJudi Minium
May 10, 2012·The Journal of Clinical Endocrinology and Metabolism·Frank GonzálezHilary E Blair
Feb 11, 2014·Trends in Endocrinology and Metabolism : TEM·Hideto KojimaLawrence Chan
Sep 1, 2015·European Journal of Endocrinology·Victor M VictorAntonio Hernandez Mijares
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Nov 7, 2013·TheScientificWorldJournal·Cuneyt KucurFatih Oghan
Feb 14, 2012·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Frank GonzálezJudi Minium
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Jul 20, 2007·BJOG : an International Journal of Obstetrics and Gynaecology·D Siassakos, P Wardle
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