In vitro evidence that phytanic acid compromises Na(+),K(+)-ATPase activity and the electron flow through the respiratory chain in brain cortex from young rats

Brain Research
Estela Natacha Brandt BusanelloM Wajner

Abstract

Phytanic acid (Phyt) tissue concentrations are increased in Refsum disease and other peroxisomal disorders characterized by neurologic damage and brain abnormalities. The present work investigated the in vitro effects of Phyt, at concentrations found in these peroxisomal disorders, on important parameters of energy metabolism in brain cortex of young rats. The parameters analyzed were CO(2) production from labeled acetate and glucose, the activities of the citric acid cycle enzymes citrate synthase, aconitase, isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase, succinate dehydrogenase, fumarase and malate dehydrogenase, as well as of the respiratory chain complexes I-IV, creatine kinase and Na(+),K(+)-ATPase. Our results show that Phyt did not alter citric acid cycle enzyme activities, or CO(2) production from acetate, reflecting no impairment of the functionality of the citric acid cycle. In contrast, respiratory chain activities were reduced at complexes I, II, I-III, II-III and IV. Membrane synaptical Na(+),K(+)-ATPase activity was also reduced by Phyt, with no alteration of creatine kinase activity. Considering the importance of the electron flow through the respiratory chain for brain energy metabolism (oxidative ...Continue Reading

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Citations

Oct 20, 2012·The Cerebellum·Estela Natacha Brandt BusanelloMoacir Wajner
Feb 5, 2013·Progress in Lipid Research·Matthew D LloydTimothy J Woodman
Aug 25, 2015·Chemico-biological Interactions·Md Torequl IslamRivelilson Mendes de Freitas
Jul 1, 2016·Molecular Neurobiology·Rodrigo Binkowski de AndradeClovis Milton Duval Wannmacher

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