In vitro evidence that RNA Polymerase acetylation and acetyl phosphate-dependent CpxR phosphorylation affect cpxP transcription regulation

FEMS Microbiology Letters
Bruno P LimaAlan J Wolfe

Abstract

The central metabolite acetyl phosphate (acP) has long been proposed to influence transcription regulation by directly transferring its phosphoryl group to a number of response regulators in many bacterial species. Here, we provide in vitro evidence for this proposition and demonstrate, using an in vitro transcription system, that acP-dependent phosphorylation of aspartate 51 of CpxR induces transcription of one of its regulon members in E. coli, cpxP. We also used this in vitro transcription system to extend our previously reported in vivo data that hypothesized that acetylation of RNA polymerase (RNAP) influences acP-dependent cpxP transcription, using glutamine as a genetic mimic for acetylated arginine 291 of the carboxy-terminal domain of RNAP α subunit. The data we present here lend strong support to the hypothesis that acP has a direct effect on transcription regulation in E. coli via phosphorylation of CpxR, and that RNAP acetylation can modulate this response.

References

Feb 24, 1998·Journal of Bacteriology·P N Danese, T J Silhavy
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Apr 16, 2002·The Journal of Biological Chemistry·Peter De WulfEdmund C C Lin
Nov 4, 2004·The Journal of Biological Chemistry·Kaneyoshi YamamotoAkira Ishihama
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Alan J Wolfe
Jul 25, 2006·Bioscience, Biotechnology, and Biochemistry·Kaneyoshi Yamamoto, Akira Ishihama
Jan 29, 2008·Journal of Bacteriology·Alan J WolfeBozena Zemaitaitis
Sep 3, 2008·Molecular Microbiology·Albert Siryaporn, Mark Goulian
Dec 24, 2008·Journal of Bacteriology·Nancy L Price, Tracy L Raivio
May 19, 2009·Journal of Molecular Biology·Eli S GrobanChristopher A Voigt
Oct 31, 2009·Chemistry & Biology·Kinga Kamieniarz, Robert Schneider
Feb 2, 2010·Current Opinion in Microbiology·Alan J Wolfe
Feb 13, 2010·Current Opinion in Microbiology·Mark Goulian
Mar 12, 2010·Current Opinion in Microbiology·Robert B Bourret, Ruth E Silversmith
Jul 4, 2012·Annual Review of Microbiology·Emily J Capra, Michael T Laub
Jul 26, 2012·The Journal of Biological Chemistry·Bruno P LimaAlan J Wolfe
Dec 19, 2013·Frontiers in Microbiology·Allison N Norsworthy, Karen L Visick

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Citations

Apr 26, 2017·Journal of Bacteriology·Valerie J Carabetta, Ileana M Cristea

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