In vitro evolution of recognition specificity mediated by SH3 domains reveals target recognition rules.

The Journal of Biological Chemistry
Simona PanniGianni Cesareni

Abstract

We have designed a repertoire of 10(7) different SH3 domains by grafting the residues that are represented in the binding surfaces of natural SH3 domains onto the scaffold of the human Abl-SH3 domain. This phage-displayed library was screened by affinity selection for SH3 domains that bind to the synthetic peptides, APTYPPPLPP and LSSRPLPTLPSP, which are peptide ligands for the human Abl or Src SH3 domains, respectively. By characterizing the isolates, we have observed that as few as two or three amino acid substitutions lead to dramatic changes in recognition specificity. We propose that the ability to shift recognition specificity with a small number of amino acid replacements is an important evolutionary characteristic of protein binding modules. Furthermore, we have used the information obtained by these in vitro evolution experiments to generate a scoring matrix that evaluates the probability that any SH3 domain binds to the peptide ligands for the Abl and Src SH3 domains. A table of predictions for the 28 SH3 domains of baker's yeast is presented.

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Citations

Jan 28, 2003·Current Opinion in Chemical Biology·Sachdev S SidhuCharles Boone
Oct 8, 2005·Nature Biotechnology·H Kaspar BinzAndreas Plückthun
Apr 15, 2011·The Journal of Biological Chemistry·Maryna GorelikAlan R Davidson
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Nov 1, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elena G Arias-SalgadoSanford J Shattil
Jul 12, 2011·Biotechnology Advances·Martina CarducciGianni Cesareni
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