In Vitro Examination of the Thrombolytic Efficacy of Desmoteplase and Therapeutic Ultrasound Compared with rt-PA

Ultrasound in Medicine & Biology
Florian C RoesslerJürgen Eggers

Abstract

The aim of the study described here was to evaluate the thrombolytic efficacy of combined treatment with the fibrin-selective plasminogen activator desmoteplase (DSPA) and therapeutic ultrasound (sonothrombolysis [STL]) compared with conventional rt-PA (recombinant tissue plasminogen activator) treatment in vitro. Lysis rates were determined by the weight loss of platelet-rich plasma (PRP) clots treated with rt-PA (60 kU/mL) or DSPA (2 μg/mL) combined with pulsed wave ultrasound (2 MHz, 0.179 W/cm(2)). To reveal the individual effects of medication and ultrasound, lysis rates were also determined for DSPA monotherapy and for combined treatment with rt-PA and ultrasound. Clots solely placed in plasma served as the control group. Lysis increased significantly with rt-PA (26.5 ± 7.8%) and DSPA (30.5 ± 6%) compared with the control group (18.2 ± 5.9%) (each p < 0.001). DSPA lysis was more effective than rt-PA lysis (without STL: p = 0.015, with STL: p = 0.01). Combined treatment with DSPA and 2-MHz STL significantly exceeded rt-PA lysis (32.8% vs. 26.5%, p < 0.001).

References

Dec 4, 1992·Annals of the New York Academy of Sciences·W D SchleuningW Witt
Jan 1, 1995·Ultrasound in Medicine & Biology·C W FrancisC Cox
Jan 1, 1996·Ultrasound in Medicine & Biology·C X DengC K Holland
May 13, 1999·Ultrasound in Medicine & Biology·S BehrensM Hennerici
Apr 27, 2001·Echocardiography·C W Francis
Feb 8, 2003·Stroke; a Journal of Cerebral Circulation·Gabriel T LiberatoreRobert L Medcalf
Nov 19, 2004·The New England Journal of Medicine·Andrei V AlexandrovUNKNOWN CLOTBUST Investigators
Mar 23, 2005·Neurology·Jürgen EggersInke R König
Apr 1, 2006·Stroke; a Journal of Cerebral Circulation·Anthony J FurlanUNKNOWN DEDAS Investigators
Feb 3, 2007·Stroke; a Journal of Cerebral Circulation·Joung-Ho Rha, Jeffrey L Saver
Apr 17, 2007·Ultrasound in Medicine & Biology·Adrian F ProkopRonald A Roy
Sep 15, 2007·Thrombosis Research·Christy K HollandGeorge J Shaw
Nov 13, 2008·Physics in Medicine and Biology·Azita SoltaniDoulas R Hansmann
Dec 2, 2008·Ultrasound in Medicine & Biology·Jürgen EggersGünter Seidel
May 22, 2009·Expert Opinion on Biological Therapy·Maurizio PaciaroniJulien Bogousslavsky
Jul 5, 2011·Ultrasound in Medicine & Biology·Kathryn E HitchcockChristy K Holland
Apr 5, 2012·Stroke; a Journal of Cerebral Circulation·Jochen B FiebachSalvador Pedraza
May 1, 2012·Ultrasound in Medicine & Biology·Bénédicte PetitEric Allémann
Aug 14, 2012·International Journal of Stroke : Official Journal of the International Stroke Society·Deena M NasrAlejandro A Rabinstein
Sep 17, 2013·Journal of Neurology·Réza Behrouz
Sep 7, 2014·Journal of Neuroscience Methods·Florian C RoesslerGünter Seidel

❮ Previous
Next ❯

Citations

Oct 31, 2018·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Katrin VoelterSimon Anton Pot
Dec 12, 2021·European Journal of Drug Metabolism and Pharmacokinetics·Bartlomiej Piechowski-JozwiakJulien Bogousslavsky

❮ Previous
Next ❯

Related Concepts

Related Feeds

Acute Stroke

A stroke occurs when blood supply to the brain is interrupted depriving the brain of oxygen and nutrients. This feed focuses cerebrovascular accidents including ischemic and paralytic stroke.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.