PMID: 20650062Feb 1, 1995Paper

In Vitro expression of drug metabolizing enzyme activities in human adult keratinocytes under various culture conditions and their response to inducers

Toxicology in Vitro : an International Journal Published in Association with BIBRA
B HirelA Guillouzo

Abstract

In this study we analysed the expression and induction of several drug metabolizing enzymes involved in either phase I or phase II reactions, in adult human keratinocytes cultured in submerged conditions. We also evaluated the influence of confluence, subcultivation and cryopreservation on the expression of these enzymes. Besides ethoxyresorufin-O-deethylase (EROD) and glutathione S-transferase (GST) activities, which have been shown previously to be maintained in such cultures, three additional enzyme activities were measured (i.e. phenacetin deethylase, a phase I enzyme, and procainamide N-acetyltransferase and paracetamol sulfotransferase, two phase II enzymes). Post-confluent keratinocytes showed decreased activities in comparison with preconfluent cells and the different enzymes tested revealed different patterns. After confluence, some activities, such as those of procainamide N-acetyltrans-ferase, phenacetin deethylase and paracetamol sulfotransferase, showed only a slight decrease, whereas EROD and GST activities were decreased by 65 and 50%, respectively. No major differences were observed between keratinocytes in primary culture and those in second subculture. After freezing, xenobiotic metabolizing enzyme activities ...Continue Reading

References

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Citations

Aug 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Patoux-PibouinA Guillouzo
Mar 21, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T MossF M Williams
Mar 15, 2001·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·M Sandbacka, B Isomaa
Nov 8, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A GelardiM Ferro
Jun 19, 1998·Chemico-biological Interactions·P WolkensteinP Beaune
Dec 7, 2007·Drug Metabolism Reviews·Franz OeschRobert Landsiedel
May 1, 1996·The British Journal of Dermatology·B HirelA Guillouzo

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