Abstract
A cell wall extract from the gram-positive bacterium Actinomyces viscosus contains the mitogen AVIS, a potent polyclonal B-cell activator for murine B lymphocytes. Cultures of splenocytes from heterozygous nude mice in the presence of an optimal concentration of AVIS responded by a deoxyribonucleic acid synthesis response, and proliferaction reached maximal levels after 3 to 4 days. There was no requirement for T cells in the deoxyribonucleic acid synthesis, proliferactive, immunoglobulin M (IgM), or IgG responses. Significant numbers of IgM-producing cells were present as early as day 2 of culture, whereas later in the culture periods (days 3 to 6) IgG-producing plasmablasts and plasma cell were observed. In cultures of splenocytes from nude mice stimulated with AVIS for 4 to 5 days, 20 to 25% of the recoverable cells synthesized IgM, and 10% contained only IgG2 or IgG3; 5 to 8% of the cells stained for both IgM and IgG2 or both IgM and IgG3. Fine-structure analysis of AVIS-stimulated splenocytes from heterozygous nude mice after 3 days of culture demonstrated that 20 to 25% of the cells were activated to various degrees. Of most importance, all of the activated cells had the characteristic of B lymphoblasts, plasmablasts, or ...Continue Reading
References
Sep 1, 1975·Scandinavian Journal of Immunology·E Gronowicz, A Coutinho
Apr 1, 1978·Developmental and Comparative Immunology·J A Clagett, D Engel
Jan 1, 1977·The Journal of Experimental Medicine·D PrimiG Möller
Jul 1, 1978·The Journal of Experimental Medicine·C BonaR G Miller
Jan 1, 1976·Scandinavian Journal of Immunology·E Gronowicz, A Coutinho
May 20, 1976·Nature·G Biberfeld, E Gronowicz
Jan 1, 1976·Scandinavian Journal of Immunology·E GronowiczA Coutinho
Dec 1, 1974·The Journal of Experimental Medicine·J A ClagettW O Weigle
Dec 1, 1974·Journal of Immunological Methods·T Asantila, P Toivanen
Oct 1, 1973·The Journal of Experimental Medicine·M P ScheidA L Goldstein
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C BonaL Chedid
Dec 13, 1972·Nature: New Biology·B M Sultzer, B S Nilsson
Dec 1, 1972·Bacteriological Reviews·K H Schleifer, O Kandler
Mar 1, 1974·The Journal of Experimental Medicine·J A Clagett, W O Weigle
Nov 1, 1974·European Journal of Immunology·E Gronowicz, A Coutinho
Nov 5, 1971·Biochemical and Biophysical Research Communications·J Lenard
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·J H LUFT
Apr 1, 1965·The Journal of Cell Biology·J M FRASCA, V R PARKS
Citations
Aug 1, 1982·Japanese Journal of Medical Science & Biology·S HamadaJ R McGhee
Oct 19, 2005·Brazilian Oral Research·Luciane Dias de OliveiraAntonio Olavo Cardoso Jorge
Sep 1, 1987·Journal of Periodontal Research·Y HaraM Aono
Mar 1, 1984·Journal of Dental Research·M A TaubmanC L Olson
Jan 1, 1988·Journal of Periodontal Research·H ItoH Okada
Mar 1, 1988·Journal of Periodontal Research·Y HaradaH Okada
Apr 1, 1983·Scandinavian Journal of Immunology·S KimuraJ R McGhee
Apr 1, 1984·Immunological Reviews·S Bergstedt-LindqvistE Severinson
Jan 14, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·N Takahashi, T Yamada
Nov 1, 1988·Advances in Dental Research·H OkadaY Harada
Mar 23, 2007·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Luciane Dias de OliveiraMarcia Carneiro Valera
Jan 1, 1981·Infection and Immunity·D F Mangan, D E Lopatin
Nov 1, 1982·Infection and Immunity·R M McCarronD C Birdsell