In vitro extracellular matrix accumulation of nasal and articular chondrocytes for intervertebral disc repair

Tissue & Cell
Srujana Vedicherla, Conor T Buckley

Abstract

Chondrocyte based regenerative therapies for intervertebral disc repair such as Autologous Disc Cell Transplantation (ADCT, CODON) and allogeneic juvenile chondrocyte implantation (NuQu®, ISTO Technologies) have demonstrated good outcomes in clinical trials. However concerns remain with the supply demand reconciliation and issues surrounding immunoreactivity which exist for allogeneic-type technologies. The use of stem cells is challenging due to high growth factor requirements, regulatory barriers and differentiation towards a stable phenotype. Therefore, there is a need to identify alternative non-disc cell sources for the development and clinical translation of next generation therapies for IVD regeneration. In this study, we compared Nasal Chondrocytes (NC) as a non-disc alternative chondrocyte source with Articular Chondrocytes (AC) in terms of cell yield, morphology, proliferation kinetics and ability to produce key extracellular matrix components under 5% and 20% oxygen conditions, with and without exogenous TGF-β supplementation. Results indicated that NC maintained proliferative capacity with high amounts of sGAG and lower collagen accumulation in the absence of TGF-β supplementation under 5% oxygen conditions. Importa...Continue Reading

Citations

Sep 7, 2019·Current Stem Cell Research & Therapy·Ying Chen, Liling Tang
Mar 4, 2020·Tissue Engineering. Part a·Matthew Anderson-BaronAdetola B Adesida
Dec 14, 2019·Current Stem Cell Research & Therapy·Zohreh BagherAlexander Seifalian
May 23, 2018·JOR Spine·Sarah E GullbrandRobert L Mauck
Jun 7, 2020·Cellular and Molecular Life Sciences : CMLS·Tianyou LiMing Pei
Jan 16, 2021·International Journal of Molecular Sciences·Laura BaumgartnerJérôme Noailly

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Related Papers

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Srujana Vedicherla, Conor T Buckley
European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
Sarah TurnerSally Roberts
© 2022 Meta ULC. All rights reserved