In vitro fabrication of tendon substitutes using human mesenchymal stem cells and a collagen type I gel

Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft für Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft für Mikrochirurgie der Peripheren Nerven und Gefässe : Organ der Vereinigung der Deut
S KallP M Vogt

Abstract

Reconstruction of tendon tissue is problematic in many cases. Since direct tendon suture is often impossible, major reconstruction with the use of free tendon transplants or tendon transposition is necessary. Important motor units often have to be sacrificed for reconstructive purposes. In this study we investigated whether long tendon-like substitutes can be fabricated in vitro from human mesenchymal stem cells (MSCs) and a collagen type I gel when cultured under cyclic stretching conditions. MSCs were obtained from bone marrow aspirates of the iliac crest. Cells were suspended in a collagen type I gel and polymerized in a glass-cylinder with defined size. The fabricated tendon substitutes underwent static stretching for 14 days followed by cyclic stretching for 21 days in a special manufactured bioreactor. Non-stretched substitutes served as a control. Macroscopically the stretched tendon substitutes showed an increased opacity and a smoother surface structure compared to the non-stretched control. The stretched substitutes displayed more spindle-shaped, longitudinal orientated cells, a tendon-like organization of the collagen matrix, and a parallel organization of the collagen fibers when stained with Hematoxylin/Eosin and E...Continue Reading

Citations

Apr 2, 2005·Der Chirurg; Zeitschrift für alle Gebiete der operativen Medizen·H-G Machens, P Mailänder
Mar 19, 2011·Tissue Engineering. Part C, Methods·Jeffrey A PatenJeffrey W Ruberti
Jan 1, 2008·Indian Journal of Plastic Surgery : Official Publication of the Association of Plastic Surgeons of India·Bassetto FrancoFrancesco Mazzoleni
Jun 18, 2009·Orvosi hetilap·Antal Salamon, Erzsébet Toldy
Aug 18, 2009·Journal of Tissue Engineering and Regenerative Medicine·Hallie E BrinkSteven B Nicoll
Feb 27, 2010·Journal of Biomedical Materials Research. Part a·Serafim M OliveiraCristina C Teixeira

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