In vitro fermentation of copra meal hydrolysate by human fecal microbiota

3 Biotech
Phatcharin PrayoonthienSuttipun Keawsompong

Abstract

Copra meal hydrolysate (CMH) is obtained by hydrolyzing defatted copra meal with β-mannanase from Bacillus circulans NT 6.7. In this study, we investigated the resistance of CMH to upper gastrointestinal tract digestion and the fecal fermentation profiles of CMH. Fecal slurries from four healthy human donors were used as inocula, and fructooligosaccharides (FOS) were used as a positive prebiotic control. Fecal batch cultures were performed at 37 °C under anaerobic conditions. Samples were collected at 0, 10, 24 and 34 h for bacterial enumeration via fluorescent in situ hybridization and organic acid (OA) analysis. In vitro gastric stomach and human pancreatic α-amylase simulations demonstrated that CMH was highly resistant to hydrolysis. Acetate was the main fermentation product of all the substrates. The proportions of acetate production of the total OAs from FOS, CMH and yeast mannooligosaccharides (MOS) after 34 h of fermentation did not significantly differ (69.76, 65.24 and 53.93%, respectively). At 24 h of fermentation, CMH promoted the growth of Lactobacillus and Bifidobacterium groups (P < 0.01) and did not significantly differ from the results obtained using FOS. The results of in vitro fecal fermentation of CMH indica...Continue Reading

References

Dec 16, 1998·The British Journal of Nutrition·S SalminenI Rowland
Aug 14, 2002·Journal of Applied Microbiology·E Olano-MartinR A Rastell
Jul 4, 2003·Applied and Environmental Microbiology·Georgina L HoldHarry J Flint
Sep 12, 2003·Biotechnology Letters·Y Y FengW J Ng
Jul 6, 2006·Trends in Ecology & Evolution·Les DethlefsenDavid A Relman
Dec 21, 2006·Journal of Agricultural and Food Chemistry·María Luz SanzRobert A Rastall
May 27, 2008·Applied and Environmental Microbiology·G MandalariA Narbad
Feb 12, 2010·FEMS Microbiology Letters·Giuseppina MandalariMartin S J Wickham
Jun 15, 2011·Applied and Environmental Microbiology·Shahrul R SarbiniRobert A Rastall
Oct 16, 2012·Applied and Environmental Microbiology·Evelina KulcinskajaHenrik Stålbrand
Dec 10, 2013·Journal of Applied Microbiology·L De VuystF Leroy
Sep 11, 2014·Journal of Agricultural and Food Chemistry·Belén GómezJosé L Alonso
Nov 18, 2015·Carbohydrate Polymers·Patrícia MonizFlorbela Carvalheiro
Jul 10, 2016·Journal of Molecular Biology·Darrell W Cockburn, Nicole M Koropatkin

❮ Previous
Next ❯

Citations

Jul 11, 2021·International Journal of Biological Macromolecules·Suzhen LeiHongliang Zeng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.