In Vitro fertilization failure of normozoospermic men: search for a lack of testicular isozyme of angiotensin-converting enzyme

Basic and Clinical Andrology
Selima Fourati Ben MustaphaJ Mandelbaum

Abstract

Angiotensin converting enzyme (ACE) is a metalloprotease with two isoforms. The somatic isoform is a key component of the renin-angiotensin system; its main function is to hydrolyse angiotensin I into angiotensin II. The germinal or testicular isoform (tACE) located at the plasma membrane of the spermatozoa, plays a crucial role in the spermatozoa-oocyte interaction during in vivo fertilization, in rodents. Disruption of the tACE in mice has revealed that homozygous male tACE-/- sire few pups despite mating normally. Few spermatozoa from these tACE-/- mice are bound to the zona pellucida (ZP) despite normal semen parameters. Based on these findings in mice models, we hypothesized that some infertile men that have the same phenotype as the tACE-/- mice, ie normal semen parameters and a lack of sperm bind to the ZP in vitro, may have a tACE defect. Twenty four men participated to this study. The case subjects (n = 10) had normal semen parameters according to the WHO guidelines (WHO 1999) but a total in vitro fertilization failure with absence of sperm fixation to the ZP. The control subjects (n = 14) also had normal semen parameters and a normal fertilization rate ≥65%. We investigated the tACE expression in spermatozoa by Wester...Continue Reading

References

Apr 1, 1977·Physiological Reviews·M J Peach
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·F SoubrierP Corvol
Jan 1, 1995·Methods in Enzymology·P CorvolF Soubrier
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·J R HagamanD A O'Brien
Jun 1, 2000·The Journal of Biological Chemistry·S P KesslerG C Sen
Sep 14, 2001·International Journal of Andrology·H ShibaharaI Sato
Jul 15, 2003·Current Opinion in Obstetrics & Gynecology·Neal G Mahutte, Aydin Arici
Nov 5, 2005·Nature Medicine·Sebastien FuchsKenneth E Bernstein

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Methods Mentioned

BETA
gene knockout
Protein Assay
electrophoresis
PCR

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