In vitro fertilization in the pigtailed macaque (Macaca nemestrina)

Theriogenology
H Michael KubischMarion S Ratterree

Abstract

The objective of this study was to investigate the possibility of collecting oocytes and semen from pigtailed macaques (Macaca nemestrina) and to establish a protocol for the production of viable embryos that would be suitable for transfer into surrogate females. A total of 82 oocytes were collected from a total of four females (on 2 d with two females each). Semen was collected from the same male on both occasions with respective ejaculate volumes of 0.55 and 0.1 mL containing 2 x 10(9) and 6.6 x 10(8)sperm/mL. Following insemination and after 48 h in culture, 42 (51.2%) of the oocytes had cleaved. Of these, 21 were selected based on developmental stage and their morphology and cryopreserved. The remainder was kept in culture for an additional 5 d, at which time three had reached the expanded blastocyst stage. A total of five transfers were performed with frozen-thawed embryos; two of these resulted in pregnancies and the birth of infants. The results of this study demonstrated that oocytes can be retrieved from pigtailed macaques and that such oocytes can be inseminated and cultured in vitro to the blastocyst stage and give rise to viable offspring after transfer into surrogate females.

References

Jun 1, 1977·Journal of Steroid Biochemistry·P H Pétra, H S Schiller
Apr 1, 1990·Biology of Reproduction·S E LanzendorfD P Wolf
Aug 1, 1989·Biology of Reproduction·D P WolfR L Stouffer
Mar 1, 1985·Journal of in Vitro Fertilization and Embryo Transfer : IVF·J V Van der MerweP Hurter
May 1, 1974·Journal of Comparative and Physiological Psychology·G G Eaton, J A Resko
Jan 1, 1965·Folia primatologica; international journal of primatology·R E KuehnR K Morrill
Jul 1, 1984·Journal of Reproduction and Fertility·D E Boatman, B D Bavister
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B D BavisterS G Eisele
May 1, 1983·Biology of Reproduction·B D BavisterM W Vernon
Nov 26, 1998·Reviews of Reproduction·L E YoungI Wilmut
Aug 31, 2001·Human Reproduction·R R YeomanD P Wolf
Apr 12, 2002·American Journal of Primatology·James C HaBennett Dyke
Apr 1, 1963·Proceedings of the Society for Experimental Biology and Medicine·L MASTROIANNI, W A MANSON
Mar 9, 2006·Theriogenology·H M KubischM S Ratterree

❮ Previous
Next ❯

Citations

Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Qiang SunJoe Z Tsien
Aug 27, 2011·Infectious Diseases in Obstetrics and Gynecology·Jason D BellDorothy L Patton
Apr 19, 2012·American Journal of Primatology·Yongchang ChenWeizhi Ji
Mar 9, 2006·Theriogenology·H M KubischM S Ratterree

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.