In vitro fertilization of in vitro matured canine oocytes using frozen-thawed dog semen

Theriogenology
Monica De los ReyesClaudio Barros

Abstract

Experiments were conducted to evaluate in vitro fertilization (IVF) of in vitro matured (IVM) bitch oocytes using dog spermatozoa frozen in three different extenders. Sperm-rich fraction from eight ejaculates of five dogs was frozen in each one of three egg yolk Tris extenders with additional: (A) 1.4 g citric acid and 0.8 g glucose; (B) 0.7 g citric acid and 3.5 g glucose; or (C) 1.4 g citric acid and 0.8 g fructose (all with 5% glycerol in 100 mL milliQ water). Thawed sperm were co-incubated with IVM bitch oocytes for 6 h. Oocytes were fixed and evaluated under an epifluorescence microscope; penetrated oocytes were defined as those having sperm heads in the perivitelline space or in the oocyte cytoplasm. Higher penetration rates (P < 0.05) were obtained in oocytes cultured with spermatozoa frozen in extenders B and C than those frozen in extender A (33.1, 34.2 and 26.4%, respectively).

References

Jun 24, 2000·Journal of Reproduction and Fertility·B Ström HolstH Rodriguez-Martinez

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Citations

Oct 31, 2007·Zygote : the Biology of Gametes and Early Embryos·S R LeeZ Y Ryoo
Nov 11, 2008·Reproduction in Domestic Animals = Zuchthygiene·S R LeeZ Y Ryoo
Sep 17, 2009·Reproduction in Domestic Animals = Zuchthygiene·M De los ReyesJ Palomino
May 26, 2018·Zygote : the Biology of Gametes and Early Embryos·Mohamed FathiMagdy R Badr

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