In vitro fertilization of ovine oocytes vitrified by solid surface vitrification at germinal vesicle stage

Cryobiology
Adel R MoawadK H S Campbell

Abstract

Cryopreservation of immature oocytes at germinal vesicle (GV) stage would provide a readily available source of oocytes for use in research and allow experiments to be performed irrespective of seasonality or other constraints. This study was designed to evaluate the recovery, viability, maturation status, fertilization events and subsequent development of ovine oocytes vitrified at GV stage using solid surface vitrification (SSV). Cumulus oocyte complexes (COCs) obtained from mature ewes were randomly divided into three groups (1) SSV (oocytes were vitrified using SSV), (2) EXP (oocytes were exposed to vitrification and warming solutions without vitrification) or (3) Untreated (control). Following vitrification and warming, viable oocytes were matured in vitro for 24h. After that, nuclear maturation was evaluated using orcein staining. Matured oocytes were fertilized and cultured in vitro for 7days. Following SSV, 75.7% 143/189 oocytes were recovered. Of those oocytes recovered 74.8%, 107/143 were morphologically normal (viable). Frequencies of in vitro maturation were significantly (P<0.01) decreased in SSV and EXP groups as compared to control. In vitro fertilization rates were significantly (P<0.01) decreased in SSV (39.3%)...Continue Reading

References

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Citations

Dec 5, 2014·Reproduction, Fertility, and Development
Jul 16, 2014·Reproduction, Fertility, and Development·Sam SudhakaranSatish Kumar Adiga
Oct 16, 2013·The Journal of Reproduction and Development·Yuji HiraoKenji Naruse
Nov 25, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Elisa Caroline da Silva SantosKazuhiro Kikuchi
Jan 15, 2019·Reproduction in Domestic Animals = Zuchthygiene·Ebrahim AhmadiHassan Nazari
Feb 15, 2019·International Journal of Veterinary Science and Medicine·Jie ZhuYan-Feng Dai
Feb 15, 2019·International Journal of Veterinary Science and Medicine·Adel R MoawadKeith H S Campbell
Oct 6, 2017·Biopreservation and Biobanking·Guobo QuanQionghua Hong
Aug 22, 2021·Theriogenology·Najmeh DavoodianHossein Mehrban

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