In vitro fertilizing potential of urethral and epididymal spermatozoa collected from domestic cats (Felis catus)

Polish Journal of Veterinary Sciences
Sylwia Prochowska, Wojciech Niżański

Abstract

The aim of this study was to provide a comparative analysis of in vitro fertilizing potential of frozen-thawed urethral and epididymal feline spermatozoa. Both types of semen were collected from 7 cats and cryopreserved in liquid nitrogen. To perform in vitro fertilization, both urethral and epididymal samples from the same individual were thawed and spermatozoa were co-incubated with in vitro matured cat oocytes. Obtained embryos were cultured in vitro for 7 days in a commercial medium. Cleavage rate, morula rate and blastocyst rate were calculated. Experiment was run in 10 replicates. The examined parameters showed no significant differences between urethral and epididymal spermatozoa (p>0.05). Cleavage rate and embryo's development were highly variable between replicates, even for the different sperm samples collected from one individual. There was no significant correlation between fertilizing capacity of two types of spermatozoa collected from the same male. In this study we confirmed that cryopreserved urethral spermatozoa have equally good fertilizing potential as epididymal ones, and both can be successfully used for in vitro fertilization in cats with the use of commercial medium.

References

Aug 1, 1991·Biology of Reproduction·J A Lawitts, J D Biggers
Jun 14, 2000·Animal Reproduction Science·P F Watson
Mar 8, 2006·Reproduction in Domestic Animals = Zuchthygiene·D ZambelliS Belluzzi

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Citations

Feb 21, 2019·Reproduction in Domestic Animals = Zuchthygiene·Sylwia ProchowskaMarcin Pałys
Oct 2, 2019·Journal of Veterinary Research·Joanna KochanJózef Skotnicki
Mar 14, 2020·Theriogenology·Gaia Cecilia Luvoni, Martina Colombo

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