In vitro functional properties of the rat bladder regenerated by the bladder acellular matrix graft

The Journal of Urology
H J PiechotaEmil A Tanagho

Abstract

To assess the response of rat urinary bladder regenerated by the homologous bladder acellular matrix graft (BAMG) to in vitro electrical and pharmacologic stimuli. In Sprague-Dawley rats, partial cystectomy (>50%) was performed, followed by BAMG augmentation cystoplasty. After 4 months, organ bath studies of tissue strips in 10 were used to compare the contractility of the BAMG regenerates and the corresponding host detrusor smooth muscle. The BAMG regenerates exhibited contractile activity to electrical field stimulation and a qualitatively identical pattern of response to muscarinic, purinergic, alpha- and beta-adrenergic drug administration and nitric oxide. At 4 months after surgery, the maximum forces of contraction of the BAMG regenerates to carbachol stimulation amounted to close to 80% of the host bladder response. With electrical field stimulation, they equaled 44% and 62% of the host bladder response after 2.5 and 4 months, respectively. Histological and immunohistochemical studies confirmed the presence of receptors for neurotransmitters that these functional in vitro studies implied. The present study provides further evidence that augmentation cystoplasty with the BAMG leads to functional regeneration of the rat bl...Continue Reading

References

Mar 1, 1978·Journal of Biomedical Materials Research·A C NovickH Kambic
Nov 1, 1977·The Journal of Urology·H TaguchiK Saito
Nov 1, 1992·The Journal of Urology·M J Gleeson, D P Griffith
Oct 1, 1992·New York State Journal of Medicine·J A Niezgoda
May 1, 1992·The Journal of Urology·W S McDougal
Jul 1, 1992·Urology·J M KhouryG D Webster
Aug 1, 1992·The Journal of Urology·S B BauerA B Retik
Mar 1, 1990·Acta Physiologica Scandinavica·A MalmgrenP O Andersson
Apr 1, 1990·The Journal of Urology·R B Filmer, J R Spencer
Jan 1, 1989·The Journal of Urology·A L ChunR M Levin
Oct 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A El-Badawi, E A Schenk
May 1, 1972·The Journal of Urology·T H StanleyJ K Lattimer
Jan 1, 1983·Scandinavian Journal of Plastic and Reconstructive Surgery·N DanielsenG Lundborg
Jan 1, 1994·Urological Research·J A ValeK R Trott
Mar 1, 1997·The Journal of Urology·R S SutherlandD S Bredt
Apr 1, 1997·British Journal of Urology·M ProbstE A Tanagho
May 1, 1957·The Journal of Urology·A W BOHNE, K L URWILLER

❮ Previous
Next ❯

Citations

Feb 14, 2006·World Journal of Urology·Karl-Dietrich SievertEmil A Tanagho
Sep 28, 2006·Pediatric Surgery International·Yasuhisa UritaTakashi Ushida
Feb 14, 2006·Nature Clinical Practice. Urology·Anthony Atala
Feb 15, 2000·BJU International·M ProbstE A Tanagho
May 24, 2007·Tissue Engineering·Kazuyoshi IijimaOsamu Nishizawa
Jun 10, 2010·Tissue Engineering. Part a·Robert A AllenJennifer B Ogilvie
Feb 18, 2011·British Medical Bulletin·Anthony Atala
Apr 8, 2006·Current Opinion in Pediatrics·Anthony Atala
Feb 15, 2012·Annals of Surgery·Giuseppe OrlandoShay Soker
Jan 12, 2012·TheScientificWorldJournal·Manish N Patel, Anthony Atala
Apr 23, 2008·Pediatric Research·Anthony Atala
Apr 23, 2008·Pediatric Research·Jonathan L YamzonChester J Koh
Dec 10, 2009·Therapy·Roberto SolerAnthony Atala
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
Jul 31, 2013·Tissue Engineering. Part B, Reviews·Lujie SongAnthony Atala
May 26, 2004·Transplant Immunology·Chester J Koh, Anthony Atala
Sep 27, 2002·Seminars in Cell & Developmental Biology·Stephen F Badylak
Mar 13, 2014·Expert Opinion on Drug Discovery·Ling-Hong Tseng
Jul 16, 2005·Expert Opinion on Biological Therapy·Anthony Atala
Nov 13, 2012·The Journal of Surgical Research·Wenbiao LiaoYunhe Xiong
Jul 4, 2012·Fertility and Sterility·Anthony Atala
Mar 11, 2010·The Journal of Sexual Medicine·Qiang Fu, Yi-lin Cao
Jan 17, 2012·Journal of Pediatric Surgery·Anthony Atala
Jan 24, 2014·TheScientificWorldJournal·Hazem OrabiStéphane Bolduc
Nov 10, 2009·Current Opinion in Biotechnology·Anthony Atala
May 2, 2009·The Urologic Clinics of North America·Anthony Atala
Nov 17, 2011·International Neurourology Journal·James J YooBupwan Kim
Feb 13, 2007·The Journal of Surgical Research·Abdol-Mohammad KajbafzadehFatemeh Mahjoub
Jun 26, 2003·BJU International·W R CrossJ Southgate
Feb 12, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Anthony Atala
Aug 8, 2007·International Journal of Urology : Official Journal of the Japanese Urological Association·Akihiro KanematsuOsamu Ogawa
Apr 19, 2013·International Journal of Urology : Official Journal of the Japanese Urological Association·Yasuhiro Sumino, Hiromitsu Mimata
Oct 5, 2012·Acta Physiologica·P OchodnickyM C Michel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Bladder Carcinoma In Situ

Bladder Carcinoma In Situ is a superficial bladder cancer that occurs on the surface layer of the bladder. Discover the latest research on this precancerous condition in this feed.

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.