In vitro gene delivery by degraded polyamidoamine dendrimers

Bioconjugate Chemistry
M X TangF C Szoka

Abstract

Transfection of cultured cells has been reported using complexes between DNA and spherical cationic polyamidoamine polymers (Starburst dendrimers) that consist of primary amines on the surface and tertiary amines in the interior. The transfection activity of the dendrimers is dramatically enhanced (> 50-fold) by heat treatment in a variety of solvolytic solvents, e.g., water or butanol. Such treatment induces significant degradation of the dendrimer at the amide linkage, resulting in a heterodisperse population of compounds with molecular weights ranging from the very low (< 1500 Da) to several tens of kilodaltons. The compound facilitating transfection is the high molecular weight component of the degraded product and is denoted as a "fractured" dendrimer. Transfection activity is related both to the initial size of the dendrimer and its degree of degradation. Fractured dendrimers exhibit an increased apparent volume change as measured by an increase in the reduced viscosity upon protonation of the terminal amines as pH is reduced from 10.5 to 7.2 whereas intact dendrimers do not. Dendrimers with defective branching have been synthesized and also have improved transfection activity compared to that of the intact dendrimers. Fo...Continue Reading

References

Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·O BoussifJ P Behr
May 31, 1994·Annals of the New York Academy of Sciences·D T Curiel

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Citations

Mar 22, 2007·Pharmaceutical Research·Khee Dong EomHoon Yoo
Oct 1, 2009·Pharmaceutical Research·Abdulgader BaoumCory Berkland
Aug 19, 2011·Applied Biochemistry and Biotechnology·Mainak ChakrabortyVibha Rani
Dec 7, 2007·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·C BelmarP Martín-Duque
May 7, 2013·Colloids and Surfaces. B, Biointerfaces·Elzbieta Pedziwiatr-WerbickaMaria Bryszewska
Jan 17, 2004·International Journal of Pharmaceutics·Pasi LampelaAtso Raasmaja
Nov 26, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Marika RuponenArto Urtti
Feb 26, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dan LuoW Mark Saltzman
Feb 26, 2003·Journal of Colloid and Interface Science·Thomas TrimailleThierry Delair
Jun 14, 2003·Journal of Colloid and Interface Science·Toyoko ImaeMasahiko Okada
Aug 19, 2003·Biomaterials·David PutnamRobert Langer
Jun 14, 2002·Trends in Biotechnology·Byeongmoon Jeong, Anna Gutowska
Mar 14, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·W ZaunerA M Haines
Aug 23, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·U RungsardthongS Stolnik
Aug 23, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·C L Gebhart, A V Kabanov
Sep 11, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Marjo MännistöArto Urtti
Jan 21, 2000·Brain Research. Molecular Brain Research·S TakeuchiH Okazawa
Dec 18, 2001·Advanced Drug Delivery Reviews·R KircheisE Wagner
Sep 3, 2002·Advanced Drug Delivery Reviews·Thomas MerdanThomas Kissel
Jun 6, 2000·Advanced Drug Delivery Reviews· Mark Saltzman W, S P Baldwin
Nov 7, 2000·International Journal of Pharmaceutics·D S ShahA F Wilderspin
Oct 18, 2001·International Journal of Pharmaceutics·M D BrownI F Uchegbu
Apr 4, 2003·International Journal of Pharmaceutics·Shuying GaoJunfeng Zhang
Jun 20, 2002·Journal of Biotechnology·Holger Frey, Rainer Haag
Jun 20, 2002·Journal of Biotechnology·Jörg Dennig, Emma Duncan
Jul 8, 2000·Pharmaceutical Science & Technology Today·J D EichmanJ R Baker
Sep 28, 1999·Pharmaceutical Science & Technology Today·M Liu, J M Fréchet
Feb 9, 2012·Accounts of Chemical Research·Jean-Paul Behr
Mar 21, 2012·Accounts of Chemical Research·Juliane Nguyen, Francis C Szoka
Jul 19, 2011·Bioconjugate Chemistry·Daniel K BonnerPaula T Hammond
May 1, 2009·Bioconjugate Chemistry·Omathanu PerumalRangaramanujam M Kannan
Sep 12, 2009·Biomacromolecules·Jun ChenDavid Oupický
Nov 5, 2011·Chemistry of Materials : a Publication of the American Chemical Society·Yuan LuMark H Schoenfisch

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