PMID: 9181120Feb 1, 1997Paper

In vitro gene expression of aromatase in rat testicular cells

Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie
J Levallet, Serge Carreau

Abstract

The ability of the male gonad to convert androgens into estrogens is well known; the microsomal enzymatic complex involved in this transformation is named aromatase and is composed of a specific cytochrome P450 aromatase (P450arom) and a ubiquitous reductase. Using a highly specific RT-PCR method we have measured the amount of P450arom mRNA in purified Leydig and Sertoli cells prepared from 20, 40 and 70-80 day-old rats. The amount of P450arom mRNA in the Leydig cells is independent of age (40 x 10(-3) attomoles/micrograms of total RNA); in contrast, in the immature rat Sertoli cells, after 5 days of culture the amount of P450arom mRNA is 20-fold lower when compared to that of 20-day-old rat Sertoli cells (71 x 10(-3) attomoles/micrograms of total RNA). Nevertheless, irrespective of the age, the addition of either FSH or dbcAMP for 6 h increases the level of P450arom mRNA in the rat Sertoli cell preparations. Therefore, we evidenced that during testicular maturation not only the Leydig cells but also the Sertoli cells of the rat have the capacity to express the gene for cytochrome P450 aromatase.

References

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Citations

Mar 6, 2009·Microscopy Research and Technique·Serge CarreauIsabelle Galeraud-Denis
Aug 12, 1999·International Journal of Andrology·S CarreauJ Levallet
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Serge Carreau, Rex A Hess
Feb 10, 2012·Spermatogenesis·Serge CarreauChristelle Delalande
May 26, 2017·Physiological Reviews·Paul S CookeRex A Hess
Jun 22, 2007·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S CarreauC Delalande
Jun 19, 2001·Molecular and Cellular Endocrinology·R A HessJ Bahr
Nov 19, 2003·The Journal of Steroid Biochemistry and Molecular Biology·S BourguibaS Carreau

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