PMID: 8614628Apr 1, 1996Paper

In vitro generation of a circular exon from a linear pre-mRNA transcript

Nucleic Acids Research
C SchindewolfH Domdey

Abstract

Recent findings have firmly established the existence of circular exons in vivo. We were interested in the possible splicing mechanism by which these unusual mRNA molecules could be created in vitro, though no biological relevance has been attached to their existence as yet. In this report we demonstrate that a modified synthetic linear yeast ACT1 transcript whose sequence begins with the 3'-part of its original intron, is continued by 247 nt of exon sequence and terminates with the 5'-part of its intron will generate a circular exon when introduced to standard in vitro splicing reactions in whole cell splice extracts from Saccharomyces cerevisiae. The formation of a circular exon was found to be independent of specific circular or secondary structures of the pre-mRNA transcript. We hypothesize that circular exons which are found in vivo may be generated from pre-mRNAs which derive from rare events of transcription initiation within an intron.

References

Mar 1, 1992·The EMBO Journal·C CocquerelleB Bailleul
Nov 1, 1992·Genes & Development·D Frank, C Guthrie
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·X D FuA R Krainer
May 1, 1992·Genes & Development·A M ZahlerM B Roth
Feb 8, 1991·Cell·J M NigroB Vogelstein
Mar 1, 1991·Trends in Genetics : TIG·S W Ruby, J Abelson
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M L BirkelandE Puré
Jul 12, 1985·Science·B Ruskin, M R Green
Jun 1, 1988·Molecular and Cellular Biology·K A JarrellP S Perlman
Aug 1, 1985·Cell·D Solnick
Aug 1, 1985·Cell·M M KonarskaP A Sharp
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R Ng, J Abelson
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·E Ford, M Ares
Apr 11, 1995·Nucleic Acids Research·C A Schindewolf, H Domdey
Jan 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C CocquerelleB Bailleul
Sep 11, 1993·Nucleic Acids Research·M PuttarajuM D Been
Nov 1, 1994·The Journal of Cell Biology·B J BlencoweP A Sharp
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·P TabaczewskiI Stroynowski
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K A Jarrell

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Citations

May 23, 2006·The Journal of Heredity·Jonathan M HouseleyRuben D Artero
Feb 11, 2015·Nucleic Acids Research·Sonja Petkovic, Sabine Müller
Jun 1, 2016·Nature Reviews. Genetics·Christopher R SibleyJernej Ule
Aug 27, 2016·RNA Biology·Zvi Pasman, Mariano A Garcia-Blanco
Apr 15, 2018·Wiley Interdisciplinary Reviews. RNA·Jeremy E Wilusz
Jan 20, 2018·International Journal of Genomics·Luka BolhaDamjan Glavač
Jun 2, 2016·Development·Steven P Barrett, Julia Salzman
Aug 28, 2018·RNA Biology·Sonam Dhamija, Manoj B Menon
Jan 19, 2018·Journal of Ovarian Research·Guobo Quan, Julang Li
May 30, 2019·Cancer Biology & Therapy·Yi-Zhi WangJun-Chao Guo
Jul 26, 2019·The EMBO Journal·Ines Lucia PatopSebastian Kadener
Feb 6, 2020·Cancers·Eugenio MorelliNicola Amodio
Nov 9, 2017·Cellular and Molecular Life Sciences : CMLS·Lesca M HoldtDaniel Teupser
Nov 30, 2018·Non-coding RNA·Simranjeet KaurFlemming Pociot
Jan 13, 2017·British Journal of Cancer·Peifei LiJunming Guo
Jan 16, 2020·Biomedicines·Andrei A DeviatkinAlexander N Lukashev
Nov 6, 2020·Reproductive Biology and Endocrinology : RB&E·Yalan MaLianwen Zheng
Mar 16, 2021·Frontiers in Immunology·Xingyu ZhaiJianhong Lu
Aug 18, 2018·Journal of Molecular and Cellular Cardiology·Meng-Yuan ZhouXing-Dong Xiong
Oct 5, 2014·Genes & Development·Dongming Liang, Jeremy E Wilusz

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