In Vitro generation of Panton-Valentine leukocidin (PVL) in clinical Methicillin-Resistant Staphylococcus aureus (MRSA) and its correlation with PVL variant, clonal complex, infection type

Scientific Reports
Chuanling ZhangXu Chu

Abstract

The amount of Panton-Valentine leukocidin (PVL) is diverse among Staphylococcus aureus isolates from different geographical regions, and its significance in some infections is disputed. However, data concerning this information in China are limited. Fifty-one lukSF-PV+ methicillin-resistant Staphylococcus aureus (MRSA) isolates gathered from varying infections were used for PVL production using enzyme-linked immunosorbent assay, and the quantity was analyzed in correlation with PVL isoform, genetic background of the isolate, and disease category. All isolates generated PVL with a range of 0.43-360.87 μg/mL, of which 56.9% isolates (29/51) generated 51-200 μg/mL of PVL; 11.8% (6/51) yielded PVL more than 200 μg/mL, and the rest (31.4%, 16/51) produced PVL of ≤50 μg/mL. The amount of PVL was not related to its variant and infection type, although isolates from skin and soft tissue infection had relatively high mean and median. Clonal complex (CC) 22 isolates might be the producer of relatively high concentrations of PVL; however, the difference among CCs was not analyzed due to a small number of CC isolates. The relevance of PVL production with the infection type, toxin isoform, and genetic characteristic of isolates may vary by ...Continue Reading

References

Oct 19, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G LinaJ Etienne
Apr 9, 2005·The New England Journal of Medicine·Scott K FridkinUNKNOWN Active Bacterial Core Surveillance Program of the Emerging Infections Program Network
Apr 9, 2005·The New England Journal of Medicine·Loren G MillerBrad Spellberg
Dec 6, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Susan Boyle-Vavra, Robert S Daum
Apr 4, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Y H HuangL J Teng
Aug 19, 2007·The Journal of Hospital Infection·Anne TristanJerome Etienne
Jul 6, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D J WolterR V Goering
Oct 24, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S MoneckeR Ehricht
Jan 15, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stephanie M HamiltonDennis L Stevens
Mar 18, 2008·International Journal of Antimicrobial Agents·Graeme R Nimmo, Geoffrey W Coombs
Jul 20, 2010·Diagnostic Microbiology and Infectious Disease·Steven Y C TongPhilip M Giffard
Oct 26, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L J ShallcrossA C Hayward
Mar 12, 2011·The Journal of Antimicrobial Chemotherapy·Jae-Hoon SongUNKNOWN ANSORP Study Group
Oct 30, 2012·The Lancet Infectious Diseases·Laura J ShallcrossAndrew C Hayward
May 20, 2015·Frontiers in Microbiology·Shima M AbdulgaderMamadou Kaba

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Citations

Oct 16, 2018·Frontiers in Microbiology·Hefa Mangzira KemungLearn-Han Lee
Oct 27, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Raiane Cardoso ChamonKátia Regina Netto Dos Santos
Jan 29, 2021·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Ying LiZhenjiang Yao

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Methods Mentioned

BETA
ELISA
enzyme-linked immunosorbent assay
PCR
bronchoalveolar lavage

Software Mentioned

SPSS

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