In vitro genotoxic effects of titanium dioxide nanoparticles (n-TiO2 ) in human sperm cells

Molecular Reproduction and Development
Marianna SantonastasoLucia Rocco

Abstract

Titanium dioxide nanoparticles (TiO2 -NPs) are one of the most widely engineered nanoparticles used. The study has been focused on TiO 2 -NPs genotoxic effects on human spermatozoa in vitro. TiO 2 -NPs are able to cross the blood-testis barrier induced inflammation, cytotoxicity, and gene expression changes that lead to impairment of the male reproductive system. This study presents new data about DNA damage in human sperms exposed in vitro to two n-TiO 2 concentrations (1 µg/L and 10 µg/L) for different times and the putative role of reactive oxygen species (ROS) as mediators of n-TiO 2 genotoxicity. Primary n-TiO 2 characterization was performed by transmission electron microscopy. The dispersed state of the n-TiO 2 in media was spectrophotometrically determined at 0, 24, 48, and 72 hr from the initial exposure. The genotoxicity has been highlighted by different experimental approaches (comet assay, terminal deoxynucleotidyl transferase dUTP nick end labeling [TUNEL] test, DCF assay, random amplification of polymorphic DNA polymerase chain reaction [RAPD-PCR]). The comet assay showed a statistically significant loss of sperm DNA integrity after 30 min of exposure. Increased threshold of sperm DNA fragmentation was highlighted...Continue Reading

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Citations

Sep 24, 2020·Toxicology and Industrial Health·Mohammad Rafiq Wani, Ggha Shadab
Aug 9, 2019·Environmental Science and Pollution Research International·Mohamed M A HusseinIslam M Saadeldin
Feb 2, 2021·Molecular Reproduction and Development·Concetta IovineLucia Rocco
Mar 7, 2021·International Journal of Environmental Research and Public Health·Mehwish IftikharTiziana Cappello
Mar 20, 2021·The Science of the Total Environment·Maingredy Rodrigues SouzaThiago Lopes Rocha
Apr 17, 2021·Reproductive Sciences·Marianna SantonastasoLucia Rocco
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May 15, 2021·Drug Metabolism Reviews·Khaled HabasDiana Anderson
Aug 8, 2021·International Journal of Molecular Sciences·Mariana VassalMaria de Lourdes Pereira
Aug 18, 2020·Ecotoxicology and Environmental Safety·Yi WuZhenyu Wang
Apr 21, 2020·Ecotoxicology and Environmental Safety·Filomena MottolaLucia Rocco

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