In vitro gentamicin exposure alters caveolae protein profile in cochlear spiral ligament pericytes

Proteome Science
Elisa GhelfiRick A Rogers

Abstract

The aminoglycoside antibiotic gentamicin is an ototoxic drug and has been used experimentally to investigate cochlear damage induced by noise.We have investigated the changes in the protein profile associated with caveolae in gentamicin treated and untreated spiral ligament (SL) pericytes, specialized cells in the blood labyrinth barrier of the inner ear microvasculature. Pericytes from various microvascular beds express caveolae, protein and cholesterol rich microdomains, which can undergo endocytosis and transcytosis to transport small molecules in and out the cells. A different protein profile in transport-specialized caveolae may induce pathological changes affecting the integrity of the blood labyrinth barrier and ultimately contributing to hearing loss. Caveolae isolation from treated and untreated cells is achieved through ultracentrifugation of the lysates in discontinuous gradients. Mass spectrometry (LC-MS/MS) analysis identifies the proteins in the two groups. Proteins segregating with caveolae isolated from untreated SL pericytes are then compared to caveolae isolated from SL pericytes treated with the gentamicin for 24 h. Data are analyzed using bioinformatic tools. The caveolae proteome in gentamicin treated cells...Continue Reading

References

Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P S JatD Kioussis
Jul 1, 1987·The American Journal of Physiology·O SterkersC Amiel
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Jan 1, 1997·Audiology & Neuro-otology·M C Holley, P W Lawlor
Aug 7, 1999·Audiology & Neuro-otology·K K OhlemillerL L Dugan
Sep 25, 1999·Journal of Human Genetics·S UsamiT Yamaguchi
Nov 2, 2001·The Journal of Biological Chemistry·W SchubertM P Lisanti
Dec 18, 2001·Journal of Mass Spectrometry : JMS·J Peng, S P Gygi
Sep 12, 2002·Pharmacological Reviews·Babak RazaniMichael P Lisanti
May 14, 2003·Drugs of Today·Duane E Bates
Jun 6, 2003·Brain Research Bulletin·Yehoash Raphael, Richard A Altschuler
Aug 2, 2003·Nature Reviews. Cancer·Lucy A Carver, Jan E Schnitzer
Aug 29, 2003·Journal of the Association for Research in Otolaryngology : JARO·Shun-Ichi Imamura, Joe C Adams
Apr 21, 2004·The Journal of Cell Biology·Katja KöhlerAhmed Zahraoui
Jul 8, 2005·Nature·Lucas Pelkmans, Marino Zerial
Feb 1, 2006·Ear and Hearing·Donald HendersonBo Hua Hu
Mar 16, 2006·Bioconjugate Chemistry·Aparna ReniguntaJörg Hänze
Feb 3, 2007·Current Pharmaceutical Design·Erol Selimoglu
Feb 24, 2007·Nature Reviews. Molecular Cell Biology·Robert G Parton, Kai Simons
Mar 16, 2007·Experimental Cell Research·Elena SannaAntonella Tomassetti
Apr 4, 2007·Cell Metabolism·Shaohui Huang, Michael P Czech
Dec 17, 2008·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Xiaorui ShiAlfred L Nuttall
Mar 7, 2009·Noise & Health·Hongzhe Li, Peter S Steyger
Jul 16, 2009·Nature Reviews. Molecular Cell Biology·Harald Stenmark
Oct 2, 2009·Nature·Yuya NishidaShigeomi Shimizu
Jul 20, 2010·Hearing Research·Cheng-Yu LinYueliang L Guo
Jan 21, 2011·Physiological Reviews·Alex H Hutagalung, Peter J Novick
Apr 27, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Chia-Der LinChing-Yuang Lin
May 20, 2011·Proteomics·André KriegeskorteKarsten Becker
May 31, 2011·Frontiers in Bioscience (Scholar Edition)·Yong GuJiangang Shen
Aug 16, 2011·Developmental Cell·Annika ArmulikChrister Betsholtz

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Methods Mentioned

BETA
GTPase
dissection
Flow Cytometry
electrophoresis
GTPases
nucleotide exchange

Software Mentioned

ene O ntology en RI chment ana L ysis and visua L iz A tion
FlowJo
Proteomaps treemap
Chemidoc MP Imaging
DECODON
Proteomaps
GOrilla
R
VennDiagram R package
Sequest

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