In vitro glucuronidation of ochratoxin a by rat liver microsomes

Toxins
Zheng HanAlfons Callebaut

Abstract

Ochratoxin A (OTA), one of the most toxic mycotoxins, can contaminate a wide range of food and feedstuff. To date, the data on its conjugates via glucuronidation request clarification and consolidation. In the present study, the combined approaches of ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), UHPLC-Orbitrap-high resolution mass spectrometry (HRMS) and liquid chromatography-multiple stage mass spectrometry (LC-MS(n)) were utilized to investigate the metabolic profile of OTA in rat liver microsomes. Three conjugated products of OTA corresponding to amino-, phenol- and acyl-glucuronides were identified, and the related structures were confirmed by hydrolysis with β-glucuronidase. Moreover, OTA methyl ester, OTα and OTα-glucuronide were also found in the reaction solution. Based on these results, an in vitro metabolic pathway of OTA has been proposed for the first time.

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Citations

Oct 28, 2015·Toxins·Alexandra H Heussner, Lewis E H Bingle
Nov 11, 2016·Mycotoxin Research·K MuñozG H Degen
Oct 5, 2018·Arhiv za higijenu rada i toksikologiju·Marija KovačBojan Šarkanj
Jul 8, 2016·Toxins·Frantisek MalirJakub Toman
Sep 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Arnau VidalMarthe De Boevre
Feb 7, 2021·Toxins·Kristina HabschiedKrešimir Mastanjević

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