In vitro glycation of an endothelialized and innervated tissue-engineered skin to screen anti-AGE molecules

Biomaterials
Sébastien CadauFrançois Berthod

Abstract

Glycation is one of the major processes responsible for skin aging through induction of the detrimental formation of advanced glycation end-products (AGEs). We developed an innovative tissue-engineered skin combining both a capillary-like and a nerve networks and designed a protocol to induce continuous AGEs formation by a treatment with glyoxal. We determined the optimal concentration of glyoxal to induce AGEs formation identified by carboxymethyl-lysin expression while keeping their toxic effects low. We showed that our tissue-engineered skin cultured for 44 days and treated with 200 μm glyoxal for 31 days displayed high carboxymethyl-lysine expression, which induced a progressively increased alteration of its capillary and nerve networks between 28 and 44 days. Moreover, it produced an epidermal differentiation defect evidenced by the lack of loricrin and filaggrin expression in the epidermis. These effects were almost completely prevented by addition of aminoguanidine 1.5 mm, an anti-glycation compound, and only slightly decreased by alagebrium 500 μm, an AGE-breaker molecule. This tissue-engineered skin model is the first one to combine a capillary and nerve network and to enable a continuous glycation over a long-term cul...Continue Reading

References

Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·K IretonA L Sonenshein
Aug 1, 1993·Biomaterials·F BerthodC Collombel
Apr 1, 1993·Burns : Journal of the International Society for Burn Injuries·L GermainF A Auger
Jan 12, 1999·Mechanisms of Ageing and Development·A J BaileyL Knott
Sep 8, 2000·The Journal of Biological Chemistry·N VerzijlJ M TeKoppele
Mar 6, 2003·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Yasuhiko YamamotoHiroshi Yamamoto
Mar 29, 2003·Brain Research. Brain Research Reviews·Seiji KikuchiKunio Tashiro
Sep 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marie GingrasFrançois Berthod
Apr 9, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Pierre-Luc TremblayFrançois A Auger
Jun 24, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carolin TonelloRoberta Cortivo
Mar 7, 2006·The Journal of Gene Medicine·Tatsufumi MurakamiAkihiro Nakamura
Apr 30, 2008·Diabetes/metabolism Research and Reviews·Maya S P HuijbertsCasper G Schalkwijk
May 14, 2008·Current Pharmaceutical Design·Kazuhiro SugimotoSoroku Yagihashi
Jan 24, 2009·The International Journal of Lower Extremity Wounds·Erin A Rayment, Zee Upton
May 26, 2009·Neurobiology of Aging·Velandai SrikanthGerald Münch
Sep 23, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Danielle T Loughlin, Carol M Artlett
Jun 29, 2011·Biochimica Et Biophysica Acta·Keigo KawabataYoshinori Sugiyama
Sep 14, 2011·Nature Reviews. Neurology·Andrea M VincentEva L Feldman
Oct 25, 2011·Experimental Dermatology·Hwa-Young ParkEung Ho Choi
Jan 10, 2012·Gerontology·David R Sell, Vincent M Monnier
Mar 20, 2012·Chemistry Central Journal·Simon Asbjørn LarsenSuresh Is Rattan
Apr 17, 2012·Translational Research : the Journal of Laboratory and Clinical Medicine·Megan Jack, Douglas Wright
May 23, 2012·Lancet Neurology·Brian C CallaghanEva L Feldman
Apr 23, 2013·Life Sciences·Paulo Zoé Costa, Raquel Soares
Jun 5, 2013·Stem Cells Translational Medicine·Mathieu BlaisFrançois Berthod
Jun 12, 2013·Current Medicinal Chemistry·M ChenA W Stitt

❮ Previous
Next ❯

Citations

Jan 9, 2016·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·G NikolakisC C Zouboulis
Apr 1, 2018·Experimental Dermatology·Carine MainzerRomain Debret
Nov 11, 2018·International Journal of Molecular Sciences·Roberta Balansin RigonChristian Zoschke
Dec 23, 2020·Tissue Engineering. Part C, Methods·Stacey C SchutteSteve Davidson
Jun 16, 2021·Trends in Endocrinology and Metabolism : TEM·Afonso MalheiroLorenzo Moroni

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.