In vitro growth and carbon utilization of the green-leaved orchid Dendrobium officinale are promoted by mycorrhizal associations

Botanical Studies
Qiu-Xia WangHong Hu

Abstract

Mycorrhizal associations play a key role in the life cycle and evolutionary history of orchids. All orchids grow from extremely small seeds that are lacking in reserves, and germination and growth into an underground heterotrophic, achlorophyllous stage depend upon symbiotic fungi to provide nutrient. However, the nutritional physiology between this symbiosis and green-leaved orchids is still unclear. To understand further how these associations affect growth and carbon utilization of green orchids, the green orchids were inoculated with two symbiotic fungi isolated from the roots of a wild orchid (Dendrobium officinale) in vitro and (13)C stable isotope signature experiments were designed to analyze carbon nutrition acquisition. After two months, both fungi had formed mycorrhizal associations with the host roots. Moreover, the growth rate was more rapid for the mycorrhizal seedlings than for the non-mycorrhizal seedlings. The mycorrhizal seedlings not only absorbed more (13)C from the substrate, but also the S3-mycorrhizal seedlings assimilated more atmospheric (13)CO2 due to significantly higher effective quantum yield of photosystem II, compared with the non-mycorrhizal seedlings. These results suggested that the green orchi...Continue Reading

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BETA
PCR

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Contig Express
SPSS

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