PMID: 9588231May 20, 1998Paper

In vitro growth inhibition of Plasmodium falciparum by retinol at concentrations present in normal human serum

Acta Tropica
T M DavisJ Beilby

Abstract

To assess the in vitro effect of retinol on Plasmodium falciparum, the standard isolates 3D7, D10, W2 and K1 in continuous culture were exposed to retinol added in concentrations ranging from 10(-7) to 0.1 mumol/l. Parasite growth inhibition was assessed from 3H-hypoxanthine incorporation. Triplicate experiments were performed at physiological pH and in the case of D10, additional experiments were performed at pH 7.2 and 7.6. Final media retinol concentrations were assayed using high performance liquid chromatography. Retinol inhibited growth of both asynchronous and synchronous cultures of 3D7 and D10 and asynchronous cultures of W2 and K1. IC50 values determined from assayed media concentrations ranged from 0.2 to 3.9 mumol/l and were comparable to concentrations in normal human serum (1.0-3.0 mumol/l). IC50 values for asynchronous D10 cultures at pH 7.2 were lower than at pH 7.4 or 7.6 (0.5, 3.9 and 5.0 mumol/l, respectively); results from synchronous cultures were similar. These data suggest that P. falciparum is a retinol-sensitive parasite, especially at pH levels equivalent to those in an acidotic patient. Adjunctive retinol therapy may have a role in clinical management of malaria.

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Jan 1, 2007·Wiener klinische Wochenschrift·Bettina ExnerWalther H Wernsdorfer
Jan 1, 2008·Wiener klinische Wochenschrift·Benedikt LeyWalther H Wernsdorfer
Jan 30, 2003·Clinical Biochemistry·Adriana PabónSilvia Blair
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Aug 17, 2000·The Journal of Infectious Diseases·A H Shankar

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