PMID: 9440786Jan 24, 1998Paper

In vitro growth suppression by adenoviral transduction of p21 and p16 in squamous cell carcinoma of the head and neck: a research model for combination gene therapy

Archives of Otolaryngology--head & Neck Surgery
S R MobleyG L Clayman

Abstract

To determine the duration of expression of the cell cycle regulators p21 and p16 and the effect of these 2 genes both alone and in combination on the growth of squamous cell carcinoma of the head and neck cell lines in vitro. EXPERIMENTAL METHODS AND DESIGN: Cells were transduced via an adenoviral vector with p21 (Ad5CMV-p21), p16 (Ad5CMV-p16), or both. Western blotting was performed to determine duration of expression of the protein products of the transduced p21 and p16 genes. In vitro growth assays and cell cycle analyses were performed on transduced cells. Transduced gene products were detected up to day 12 after infection. Western blotting showed high levels of p21 and p16 in transduced cells. Growth suppression was observed in squamous cell carcinoma of the head and neck cell lines transduced with Ad5CMV-p21, Ad5CMV-p16, or both, but the combination of p21 and p16 did not achieve significantly greater growth suppression than that seen in cells transduced with Ad5CMV-p16 alone. Cell cycle analysis demonstrated that the percentage of cells arrested at G1 stage in the cells transduced with Ad5CMV-p16 was similar to that in the cells transduced with both Ad5CMV-p21 and Ad5CMV-p16. No significant in vivo growth suppression was...Continue Reading

Citations

May 12, 2000·The Laryngoscope·L L Gleich
Jun 16, 2004·International Journal of Cancer. Journal International Du Cancer·Steven W CoonDaniel L Van Dyke
Dec 16, 2004·Seminars in Oncology·Ezra E W Cohen
Jun 19, 1998·The Journal of the American Dental Association·D M WinnD V Kleinman
Sep 24, 2005·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Danling GuMonica L Zepeda
Apr 9, 2005·World Journal of Gastroenterology : WJG·Xiu-Sheng HeYan Chen
Jul 27, 2000·Annals of the New York Academy of Sciences·E A SausvilleA M Senderowicz
Jan 31, 2002·World Journal of Gastroenterology : WJG·X S HeL R Zhang
Mar 21, 2002·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Donna A WhyteEdward J Shillitoe
May 18, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marxa L FigueiredoDavid T W Wong
Dec 21, 2002·Surgical Oncology Clinics of North America·Douglas K Frank

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